Human Anti-PD-1 (HRP)

This product is a full-length Human IgG1 recombinant antibody specific to PD-1, fused at the genetic level to the HRP reporter enzyme. It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.

100 µg      |     Catalogue Number: CBT_A0062

Download: SDS / Datasheet

View Product

Contact us

Share:

PD-1, or programmed cell death protein 1, is a cell surface receptor protein that plays a crucial role in regulating the immune response in the human body. It is a type I transmembrane protein that belongs to the CD28/CTLA-4 family of immune checkpoint receptors. PD-1 is primarily expressed on the surface of activated T cells, B cells, natural killer (NK) cells, and certain other immune cell types. It is induced upon activation of these cells and serves as a mechanism to control the immune response and prevent excessive immune activation. Many tumors exploit the PD-1 pathway to evade immune detection and destruction. Tumor cells can upregulate PD-L1 to engage PD-1 on immune cells, leading to immune suppression and allowing the tumor to evade attack by the immune system.

Our PtX™ Human Anti-PD-1 (HRP) exhibits high specificity towards PD-1 and undergoes rigorous testing to ensure consistent performance and reliability. This specificity allows researchers to elucidate the specific roles of PD-1 in immunological processes. PtX™ Human Anti-PD-1(HRP) is suitable for use in Western blot and ELISA applications.

Additional information

Available Unit Size

0.1mg, 1mg, 10mg, 100mg, 200mg

Key Benefits

This product is a recombinant monoclonal antibody that offers several benefits including the following:

  • Enhanced reproducibility associated with HRP fusion. 
  • Reduced Background Noise 
  • Time and Cost Efficiency 
  • High batch-to-batch consistency 
  • No animals or animal products are used in the production of this antibody. 
  • BSA and Azide free. 

For more information on plant-made, recombinant monoclonal antibodies click here. 

Datasheet

Product Name PtX™ Human Anti-PD-1 (HRP) Recombinant Antibody
Catalogue Number CBT_A0062
Expression Host Nicotiana benthamiana plants
Clonality Monoclonal, recombinant
Species and Isotype Human IgG1
Tag None
Reporter Protein Horseradish Peroxidase (HRP)
Description This product is a full-length Human IgG1 recombinant antibody specific to PD-1, fused at the genetic level to HRP reporter enzyme. It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.
Target Name Programmed cell death protein 1 (PD-1)
Verified Applications Western blot, ELISA
Dilution Range Western blot (1: 1 000 – 1: 2 000)

ELISA (1: 1 000 – 1: 256 000)
Tested Species Reactivity     Human
Concentration                   1 mg/ml
Form Liquid
Colour Clear with a faint red tinge
Preparation Ready to use
Storage Short term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze thaw cycles.
Storage Buffer 0.1    M Phosphate Buffered Saline, pH 7.4

Preservative: 50% Glycerol
Purification Notes This product was purified using Protein A- affinity chromatography.
Purity 95 % as determined by SDS-PAGE
General Notes If for any reason the product does not perform as specified, please contact our scientific support team for assistance by emailing techsupport@capebiologix.com.

For Research Use only, unless otherwise indicated.

Images

Figure 1. ELISA Dose Response curve using PtX™ Human Anti-PD-1 (HRP) Antibody from 0.02 – 50 ng/uL to detect 50 ng Human PD1/ PDCD1 Protein (His tag). Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot of decreasing amounts of human PD-1 protein (17.4 kDa, migrates at ~41.1 kDa due to glycosylation) detected with PtX™ Human Anti-PD-1 (HRP) Antibody at 1: 2 500.

 

PtX™ | Plant-Based Expression

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single-use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.

Visit our Technology page to learn more.

Important!

  • For Research Use Only.
  • Not for diagnostic or therapeutic use.
  • Not for resale without express authorization.
  • Trademark.

Feel free to contact our trusted distribution partners directly if you want to order this product.

 

Contact our Distribution Partners

Related products

  Sign up for our newsletter

Click Here