Mouse Anti-VEGF
Mouse Anti-VEGF - Image 2

Mouse Anti-VEGF

Recombinant mouse monoclonal antibody against Vascular endothelial growth factor (VEGF). PtX™ Mouse Anti-VEGF is produced in Nicotiana benthamiana plants and used in various Western blot and ELISA applications.

100 µg      |     Catalogue Number: CBT_A0019

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Vascular endothelial growth factor (VEGF) is a signalling protein that plays a crucial role in angiogenesis, the formation of new blood vessels from pre-existing ones. It is produced by many different cell types, including endothelial cells, macrophages, and tumour cells, and is a key regulator of blood vessel growth and maintenance. The physiological functions of VEGF include promoting the proliferation and migration of endothelial cells, as well as increasing vascular permeability. VEGF also stimulates the production of nitric oxide, a potent vasodilator, which helps to increase blood flow to tissues. In addition to its role in normal tissue growth and repair, VEGF is also involved in pathological processes such as tumour angiogenesis and ocular neovascularization. VEGF has been found to be overexpressed in many types of cancer, including breast, lung, colorectal, and kidney cancer, among others. Its overexpression has been associated with increased tumour growth, invasion, and metastasis, as well as poor prognosis and resistance to chemotherapy and radiotherapy.

Additional information

Available Unit Size

0.1mg, 1mg, 10mg, 100mg, 200mg

Key Benefits

This product is a recombinant monoclonal antibody that offers several benefits including the following:

  • Batch-to-batch consistency.
  • This product is plant-made, no animals or animal products are used in the manufacture of this protein.
  • BSA and Azide free.

For more information on plant-made, recombinant monoclonal antibodies click here.

Datasheet

Product Name PtX™ Mouse Anti-Vascular Endothelial Growth Factor Recombinant Antibody
Catalogue Number CBT_A0019
Expression Host Nicotiana benthamiana plants
Clonality Monoclonal, recombinant
Species and Isotype Mouse IgG2a
Tag None
Reporter Protein None
Description This product is a full-length Mouse IgG2a recombinant antibody specific to the VEGF-165a antigen. It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens mediated infiltration.
Target Name Vascular Endothelial Growth Factor A (VEGF-165a)
Verified Applications Western blot, ELISA
Dilution Ranges Western blot (1: 5 000-1: 10 000)
ELISA (1: 20 000-1: 100 000)
Concentration 1.0 mg/ml
Form Liquid
Colour Clear
Preparation Ready to use
Storage Short term (up to one week): 2 – 8 °C
Long term: Aliquot and store at – 20 °C
Store immediately. Aliquot and avoid multiple freeze thaw cycles.
Storage Buffer 0.1   M Phosphate Buffered Saline, pH 7.4 Preservative: None
Purification Notes This product was purified using Protein A affinity chromatography.
Purity ≥ 90 % as determined by SDS-PAGE
General Notes If for any reason the product does not perform as specified, please contact our scientific support team for assistance by emailing sales@capebiologix.com.

Images

Figure 1. ELISA Dose Response curve of PtX™ Mouse Anti-VEGF Recombinant Antibody used to detect coated VEGF antigen at 0.05 ng/µl and increasing amount of antibody. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2: Western blot of decreasing amounts of commercial recombinant VEGF (17 kDa) detected using PtX™ Mouse Anti-VEGF at 1: 2 000 and an HRP conjugated anti-mouse secondary antibody.

PtX™ | Plant-Based Expression

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.

Visit our Technology page to learn more.

Important!

  • For Research Use Only.
  • Not for diagnostic or therapeutic use.
  • Not for resale without express authorization.
  • Trademark.

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