PtX™ Human Anti-HIV-1 p24 (HRP)

This product is a plant-made, recombinant monoclonal antibody which offers several benefits including the following:

• No secondary antibody step is required, saving time and cost, as well as improving test specificity.
• Reporter enzyme (HRP) is fused at the genetic level for increased reproducibility of results.
• No animals or animal products are used in the development of this protein.
• BSA and Azide free.

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Figure 1: A) SDS-PAGE analysis of PtX™ Human HIV-1 p24 Recombinant Antibody (HRP) (10 µg) under denaturing and reducing conditions. The gel was stained with Coomassie blue (Lane 2). The antibody, transferred to the membrane was detected with the reporter protein substrate. The heavy chain, full antibody, and other isoforms were visible as per blot (Lane 3). B) Dot blots for detection of HIV-1 p24 from HIV+ samples. a) serum isolated from rabbits immunized with HIV-1 vaccine expressing HIV-1 Gag and Envelope, b) – c) HIV-1 Gag p24 in cell lysate and d) – e) culture medium of RK13 cells infected with MVA-EnvM3.2 FL-IP-Gag^M . f) HIV-1 recombinant p24 antigen was used as a positive control

Figure 2: Antigen-Antibody binding assay (ELISA). An ELISA plate was coated overnight with 100 ng of HIV-1 recombinant p24 antigen per well. Detection was performed using PtX™ Human HIV-1 p24 Recombinant Antibody (HRP) was applied at a dilution of 1:2 000 and incubated for 60 minutes at 37 °C, following which a colorimetric detection substrate was used for visualization. Absorbance (OD) was read spectrophotometrically at 370 nm. Increasing amounts of HIV-1 p24 antibody were plotted against the corresponding OD units.

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.

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