PtX™ Human Anti-HIV-1 p24 (HRP)

Human Anti-HIV-1 p24 antibody is a recombinant monoclonal antibody against HIV-1 p24 capsid protein, produced in Nicotiana benthamiana plants and fused to HRP for use in immunoassays for the detection of HIV-1 p24 in a variety of biological samples.

20 µg Sample Size     |     Catalogue Number: CBT_A0003

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The structural capsid protein p24, which has a molecular weight of 24 kDa, is the most abundant viral (HIV) protein and forms part of the Gag polypeptide. It is detectable at relatively high levels in the blood during the early stages of HIV infection, making it a potential viral marker for diagnosis, blood donor screening, monitoring disease progression, and evaluation of antiretroviral therapy efficacy. Initial HIV tests only measure antibody levels, which are only detectable approximately 3–4 weeks post-infection. The p24 protein can be detected in patient blood as early as two weeks post infection, making p24 antigen particularly valuable for the diagnosis of primary HIV infection.

Additional information

Available Unit Size

0.1mg, 1mg, 10mg, 100mg, 200mg

Key Benefits

This product is a plant-made, recombinant monoclonal antibody which offers several benefits including the following:

  • No secondary antibody step is required, saving time and cost, as well as improving test specificity.
  • Reporter enzyme (HRP) is fused at the genetic level for increased reproducibility of results.
  • No animals or animal products are used in the development of this protein.
  • BSA and Azide free.

For more information on plant-made, recombinant monoclonal antibodies click here.

Datasheet

Product Name PtX™ Human HIV-1 p24 Recombinant Antibody (HRP)
Catalogue Number CBT_A0003
Expression Host Nicotiana benthamiana plants
Clonality Monoclonal, recombinant
Species and Isotype Human IgG1
Tag None
Reporter Protein Horse Radish Peroxidase (HRP)
Description This is a recombinant antibody targeted to HIV-1 p24 antigen. It is produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens mediated infiltration. It is fused at the genetic level to a Horse Radish Peroxidase (HRP) reporter enzyme.
Verified Applications Western blot (1:2 000 – 1:20 000)
ELISA (1:1 000 – 1:5 000)
Concentration 1.0 mg/ml
Form Liquid
Colour Clear to light brown/yellowish
Preparation Ready to use
Storage Short-term (up to one week): 2 – 8 °C
Long-term: Aliquot and store at – 20 °C
Store immediately. Aliquot and avoid multiple freeze thaw cycles.
Storage buffer Phosphate Buffered Saline (0.1 M), pH 7.4. Preservative: none
Purification notes This product was purified using Protein A affinity chromatography.
Purity ≥ 90 % as determined by SDS-PAGE.
General notes If for any reason the product does not to give desirable results, please contact our scientific support team for assistance.

Images

Figure 1: A) SDS-PAGE analysis of PtX™ Human HIV-1 p24 Recombinant Antibody (HRP) (10 µg) under denaturing and reducing conditions. The gel was stained with Coomassie blue (Lane 2). The antibody, transferred to the membrane was detected with the reporter protein substrate. The heavy chain, full antibody, and other isoforms were visible as per blot (Lane 3). B) Dot blots for detection of HIV-1 p24 from HIV+ samples. a) serum isolated from rabbits immunized with HIV-1 vaccine expressing HIV-1 Gag and Envelope, b) – c) HIV-1 Gag p24 in cell lysate and d) – e) culture medium of RK13 cells infected with MVA-EnvM3.2 FL-IP-GagM . f) HIV-1 recombinant p24 antigen was used as a positive control

Figure 2: Antigen-Antibody binding assay (ELISA). An ELISA plate was coated overnight with 100 ng of HIV-1 recombinant p24 antigen per well. Detection was performed using PtX™ Human HIV-1 p24 Recombinant Antibody (HRP) was applied at a dilution of 1:2 000 and incubated for 60 minutes at 37 °C, following which a colorimetric detection substrate was used for visualization. Absorbance (OD) was read spectrophotometrically at 370 nm. Increasing amounts of HIV-1 p24 antibody were plotted against the corresponding OD units.

PtX™ | Plant-Based Expression

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.

Visit our Technology page to learn more.

Important!

  • For Research Use Only.
  • Not for diagnostic or therapeutic use.
  • Not for resale without express authorization.
  • Trademark.

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