Human Anti-HIV-1 p24 (HRP)

Human Anti-HIV-1 p24 antibody is a recombinant monoclonal antibody against HIV-1 p24 capsid protein, produced in Nicotiana benthamiana plants and fused to HRP for use in immunoassays for the detection of HIV-1 p24 in a variety of biological samples.

Catalogue Number: CBT_A0003

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The structural capsid protein p24, which has a molecular weight of 24 kDa, is the most abundant viral (HIV) protein and forms part of the Gag polypeptide. It is detectable at relatively high levels in the blood during the early stages of HIV infection, making it a potential viral marker for diagnosis, blood donor screening, monitoring disease progression, and evaluation of antiretroviral therapy efficacy. Initial HIV tests only measure antibody levels, which are only detectable approximately 3–4 weeks post-infection. The p24 protein can be detected in patient blood as early as two weeks post infection, making p24 antigen particularly valuable for the diagnosis of primary HIV infection.

Additional information

Available Unit Size

0.1mg, 1mg, 10mg, 100mg, 200mg

Key Benefits

This product is a plant-made, recombinant monoclonal antibody which offers several benefits including the following:

  • No secondary antibody step is required, saving time and cost, as well as improving test specificity.
  • Reporter enzyme (HRP) is fused at the genetic level for increased reproducibility of results.
  • No animals or animal products are used in the development of this protein.
  • BSA and Azide free.

For more information on plant-made, recombinant monoclonal antibodies click here.

Datasheet

Product Name PtX™ Human Anti-HIV-1 p24 Recombinant Antibody (HRP)
Catalogue Number CBT_A0003
Expression Host Nicotiana benthamiana plants
Clonality Monoclonal, recombinant
Species and Isotype Human IgG1
Tag None
Reporter Protein Horse Radish Peroxidase (HRP)
Description This protein is a recombinant antibody targeted to HIV-1 p24 antigen. It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens mediated infiltration. It is fused at the genetic level to a Horse Radish Peroxidase (HRP) reporter enzyme.
Verified Applications Western blot (1: 2 000 – 1: 20 000)
ELISA (1: 1 000 – 1: 5 000)
Concentration 1.0 mg/ml
Form Liquid
Colour Clear to light brown/yellowish
Preparation Ready to use
Storage Short-term (up to one week): 2 – 8 °C
Long term: Aliquot and store at – 20 °C
Store immediately. Aliquot and avoid multiple freeze-thaw cycles.
Storage buffer Phosphate Buffered Saline (0.1 M), pH 7.4. Preservative: none
Purification notes This product was purified using Protein A affinity chromatography.
Purity ≥ 90 % as determined by SDS-PAGE
98.62% as determined by Mass Spectrometry
General notes If for any reason the product does not perform as specified, please contact our scientific support team for assistance.

Images

Figure 1: Antigen-Antibody binding assay (ELISA). The 96-well plate (Greiner Bio) was coated with 100 ng of HIV-1 recombinant p24 antigen (Abcam, Ab43037) overnight at 4°C. The coated antigen was detected with primary antibody, PtX™ Human HIV-1 p24 (HRP) at a 1: 2 000 dilution. Increasing amounts of PtX™ Human HIV-1 p24 (HRP) were plotted against the corresponding absorbance units.

Figure 2: Dot blots for detection of HIV-1 p24 from HIV+ samples. a) serum isolated from rabbits immunized with HIV-1 vaccine expressing HIV-1 Gag and Envelope. Detection of HIV-1 Gag p24 in cell lysate b) – c) and d) – e) culture medium of RK13 cells infected with HIV-1 (MVA-EnvM3.2 FL-IP-GagM). f) HIV-1 recombinant p24 antigen was used as a positive control.

PtX™ | Plant-Based Expression

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.

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Important!

  • For Research Use Only.
  • Not for diagnostic or therapeutic use.
  • Not for resale without express authorization.
  • Trademark.

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