Mouse Anti-IFN-Gamma

Recombinant mouse monoclonal antibody against Interferon-Gamma cytokine. This product is produced in Nicotiana benthamiana plants and is suitable for Western Blot and ELISA applications.

100 µg      |     Catalogue Number: CBT_A0012

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Interferon-Gamma (IFN-γ) is a pleiotropic cytokine and the only member of the type II IFN family, which differs structurally and functionally from other types of IFNs. IFN-γ is a homodimer synthesized by non-covalent association of two 146 amino acid subunits in an antiparallel manner, forming a mature protein of approximately 33 kDa. Antigens, mitogens and cytokines, e.g. IL-12, IL-15 and IL-18, induce production of IFN-γ by T lymphocytes and natural killer cells. Once released, it acts as macrophage activation factor through binding of IFN-γ receptor I and II and subsequent activation of the JAK/STAT pathway. The inhibitory effects of IFN-γ on viral replication were originally reported in 1965 and its role in host response to microorganisms has been well established. IFN-γ also plays an important role in cancer immune surveillance, promoting tumor recognition and immunity.

Additional information

Available Unit Size

0.1mg, 1mg, 10mg, 100mg, 200mg

Key Benefits

This product is a recombinant monoclonal antibody that offers several benefits including the following:

  • Batch-to-batch consistency.
  • No animals or animal products are used in the development of this protein.
  • BSA and Azide free.

For more information on plant-made, recombinant monoclonal antibodies click here.

Datasheet

Product Name  PtX™ Mouse Anti-Interferon-Gamma Recombinant Antibody
Catalogue Number  CBT_A0012
Expression Host Nicotiana benthamiana plants
Clonality Monoclonal, recombinant
Species and Isotype Mouse IgG2a
Tag  None
Reporter Protein None
Description Recombinant mouse monoclonal antibody against IFN-γ cytokine, produced via Agrobacterium tumefaciens infiltration of Nicotiana benthamiana plants.
Verified Applications  Western blot, ELISA.
Recommended dilutions Western blots (1: 2 000 – 1: 10 000)
ELISA (1: 1 000 – 1: 5 000)
Concentration 1.0 mg/ml
Form Liquid
Colour Clear
Preparation Ready to use
Storage Short-term (up to one week): 2 – 8 °C
Long term: Aliquot and store at – 20 °C
Store immediately. Aliquot and avoid multiple freeze-thaw cycles.
Storage buffer 0.1 M Phosphate Buffered Saline, pH 7.4.
Preservative: none
Purification notes This product was purified using Protein A affinity chromatography.
Purity ≥ 90 % as determined by SDS-PAGE.
General notes If for any reason the product does not perform as specified, please contact our scientific support team for assistance by emailing sales@capebiologix.com.

Images


Figure 1: ELISA Dose Response curve showing increasing absorbance at 370 nm with increasing amounts of antibody added to 96 well plate coated with 0.3 ng/µl of IFN antigen.

Figure 2: Western blot analysis of PtX™ Mouse Anti-IFN-Gamma. Lanes 2–5: Varying amounts of IFN-γ were run on the SDS-PAGE. Separated bands were transferred to the membrane and PtX™ Mouse Anti-IFN- Gamma (1: 2 500) was used to detect the antigen. The two bands of IFN-Gamma were visualized following the addition of anti-rabbit secondary antibody with HRP and substrate. Our antibody was able to detect antigen amounts upwards of 31 ng (Lane 5).

PtX™ | Plant-Based Expression

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single-use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.

Visit our Technology page to learn more.

Important!

  • For Research Use Only.
  • Not for diagnostic or therapeutic use.
  • Not for resale without express authorization.
  • Trademark.

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