PtX™ Spike Protein S1 (His) Ultra

Recombinant SARS-CoV-2 Spike protein (S1) is produced in Nicotiana benthamiana plants and fused to a 6X Histidine tag for use in Western blot and ELISA applications.

100 µg     |     Catalogue Number: CBT_P0003

Download: SDS / Datasheet

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The Spike Protein is the characteristic surface antigen of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), or 2019 Novel Coronavirus. Spike protein is assembled as a trimer consisting of S1 and S2 domains. SARS-CoV-2 binds to angiotensin-converting enzyme 2 (ACE2) receptors on host cell membranes by specific interaction with its receptor-binding domain (RBD), located on the S1 domain. We have recombinantly produced the S1 subunit, genetically fused to a 6X Histidine Tag. This antigen is available in 100ug research amounts, as well as bulk amounts. Our S1-His has been externally validated in publications showing it is recognized by SARS-CoV-2 positive patient serum.

Additional information

Available Unit Size

0.1mg, 1mg, 10mg, 100mg, 200mg

Key Benefits

This product is a plant-made, recombinant monoclonal antibody which offers several benefits including:

  • No animals or animal products are used in its development.
  • Proteins are fused at the genetic level for increased reproducibility of results.
  • BSA and Azide free.

For more information on plant-made, recombinant monoclonal antibodies click here.

Datasheet

Product Name SARS-CoV-2 Spike Protein S1 Recombinant Antigen (His tag) Ultra
Catalogue Number CBT_P0003
Expression Host Nicotiana benthamiana plants
Clonality N/A
Species and Isotype Human
Tag poly-histidine (His) and SEKDEL tags at the C-terminus
Reporter Protein None
Description This product is a recombinant peptide fused at the genetic level to both a 6X Histidine and a SEKDEL tag. It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens mediated infiltration.
Target name N/A
Target sequence The S1 portion of the Spike protein of SARS-CoV-2 (GenBank: MN908947.3) fused to a poly-histidine and a SEKDEL tag at the C-terminus.
Verified application Western blot
ELISA
Concentration 1.0 mg/ml
Form Liquid
Colour Clear to light yellow brown.
Preparation Ready to use
Storage Short-term (up to one week): 2 – 8 °C
Long-term: Aliquot and store at – 20 °C
Store immediately. Aliquot and avoid multiple freeze-thaw cycles.
Storage buffer 0.1  M Phosphate Buffered Saline, pH 7.4
Preservative: None
Purification notes This product was purified using Protein A affinity chromatography.
Purity ≥ 85 % as determined by SDS-PAGE
General notes If for any reason the product does not perform as specified, please contact our scientific support team for assistance by emailing sales@capebiologix.com.

Publications

  • Pietschmann J, Voepel N, Voß L, Rasche S, Schubert M, Kleines M, Krause HJ, Shaw TM, Spiegel H, Schroeper F. Development of Fast and Portable Frequency Magnetic Mixing-Based Serological SARS-CoV-2-Specific Antibody Detection Assay. Front Microbiol. 2021 May 5;12:643275. doi: 10.3389/fmicb.2021.643275. URL: https://pubmed.ncbi.nlm.nih.gov/34025604/
  • Makatsa Mohau S., Tincho Marius B., Wendoh Jerome M., Ismail Sherazaan D., Nesamari Rofhiwa, Pera Francisco, de Beer Scott, David Anura, Jugwanth Sarika, Gededzha Maemu P., Mampeule Nakampe, Sanne Ian, Stevens Wendy, Scott Lesley, Blackburn Jonathan, Mayne Elizabeth S., Keeton Roanne S., Burgers Wendy A. SARS-CoV-2 Antigens Expressed in Plants Detect Antibody Responses in COVID-19 Patients. Frontiers in Plant Science. 2021;12:589940. doi:10.3389/fpls.2021.589940. URL: https://www.frontiersin.org/articles/10.3389/fpls.2021.589940/full

 

PtX™ | Plant-based Expression

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single-use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.

Visit our Technology page to learn more.

Important!

  • For Research Use Only.
  • Not for diagnostic or therapeutic use.
  • Not for resale without express authorization.
  • Trademark.

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