Human Immunodeficiency Virus Type 2 gp36 Protein

HIV-2 gp36 protein fused to a mouse fc region tag is produced in Nicotiana benthamiana plants.

100 µg Sample Size     |     Catalogue Number: CBT_P0006

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The human immunodeficiency virus (HIV) is a lentivirus (slowly replicating retrovirus) that causes the acquired immunodeficiency syndrome (AIDS), a condition in humans in which progressive failure of the immune system allows life-threatening opportunistic infections and cancers to thrive.

HIV GP36 is a transmembrane protein located in the envelope of the virus specific to HIV-2.

Additional information

Available Unit Size

0.1mg, 1mg, 10mg, 100mg, 200mg

Key Benefits

This product is a plant-made, recombinant protein, which offers several advantages including.

  • Protein is genetically fused for increased reproducibility of results.
  • No animals or animal products are used in the development of this protein.
  • Azide and BSA free.

For more information on plant-made, recombinant monoclonal proteins click here.

Images

Figure 1. Western blot: Functional Western blot analysis to evaluate if the gp36 Fc protein can be detected by HIV-2 serum. One microgram of gp36 Fc protein under denaturing and reducing conditions was probed with HIV-2 patient serum at a 1: 2 000 dilution. Goat Anti-Human IgG (Fc-specific) HRP (Sigma Aldrich, A0170) at a 1: 5 000 dilution was used for detection. The expected protein band sizes of monomer gp36 Fc (~35 kDa) and dimer gp36 Fc (~65 kDa) were observed, with some additional non-specific bands that can be as a result of different glycoforms of the protein.

Figure 2. Indirect ELISA. ELISA analysis of gp36 Fc protein at 2 ng/μl probed with 1:200 and 1:400 dilution of patient HIV-1, HIV-2 and negative serum. A Goat Anti-Human IgG (Fc-specific) HRP (Sigma Aldrich, A0170) at a 1: 10 000 dilution was used for detection.

Datasheet

Product Name PtX™ Human Immunodeficiency Virus Type 2 gp36 Protein
Catalogue Number CBT_P0005
Expression Host Nicotiana benthamiana plants
Clonality N/A
Species Human Immunodeficiency Virus 1 (HIV-1) 
Tag Mouse IgG1 Fc Tag
Reporter Protein None
Description This product is recombinantly produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens mediated infiltration.
Verified Applications Western blot (1: 1 000-1: 2 000)
ELISA (1: 1 000-1: 5 000)
Concentration 1.0 mg/ml
Form Liquid
Colour Light reddish
Preparation Ready to use
Storage Short term (up to one week): 2 – 8 °C
Long term: Aliquot and store at – 20 °C
Store immediately. Aliquot and avoid multiple freeze thaw cycles.
Storage buffer 0.1  M Phosphate Buffered Saline, pH 7.4. Preservative: None
Purification notes This product was purified using Immobilised metal affinity chromatography (IMAC).
Purity ≥ 85 % as determined by SDS-PAGE
97.06% as determined by Mass Spectrometry
General notes If for any reason the product does not perform as specified, please contact our scientific support team for assistance by emailing sales@capebiologix.com.

PtX™ | Plant-Based Expression

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single-use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins are extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.

Visit our Technology page to learn more.

Important!

  • For Research Use Only.
  • Not for diagnostic or therapeutic use.
  • Not for resale without express authorization.
  • Trademark.

Feel free to contact our trusted distribution partners directly if you want to order this product.

 

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