Human Immunodeficiency Virus Type 1 gp41 Protein

HIV-1 gp41 protein fused to a mouse fc region tag is produced in Nicotiana benthamiana plants.

100 µg Sample Size     |     Catalogue Number: CBT_P0005

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The human immunodeficiency virus (HIV) is a lentivirus (slowly replicating retrovirus) that causes the acquired immunodeficiency syndrome (AIDS), a condition in humans in which progressive failure of the immune system allows life-threatening opportunistic infections and cancers to thrive.

Gp41 is a glycoprotein in the envelope of HIV-1. It is a subunit of the envelope protein complex of retroviruses.

Additional information

Available Unit Size

0.1mg, 1mg, 10mg, 100mg, 200mg

Key Benefits

This product is a plant-made, recombinant protein, which offers several advantages including.

  • Protein is genetically fused for increased reproducibility of results.
  • No animals or animal products are used in the development of this protein.
  • Azide and BSA free.

For more information on plant-made, recombinant monoclonal proteins click here.

Images

Figure 1. ELISA Dose Response curve showing increasing absorbance at 370 nm with increasing amounts of TX™ Recombinant Protein A (HRP) used to detect Human IgG antigen.

Figure 2. Western blot of decreasing amounts of GST antigen (28 kDa) detected with PtX Rabbit Anti-GST Recombinant Antibody at 1: 5 000 and PtX™ Recombinant Protein A (HRP) at 1: 1 000 as secondary detection probe.

Datasheet

Product Name PtX™ Human Immunodeficiency Virus Type 1 gp41 Protein
Catalogue Number CBT_P0005
Expression Host Nicotiana benthamiana plants
Clonality N/A
Species Human Immunodeficiency Virus 1 (HIV-1) 
Tag Mouse IgG1 Fc Tag
Reporter Protein None
Description This product is recombinantly produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens mediated infiltration.
Verified Applications Western blot (1: 1 000-1: 2 000)
ELISA (1: 1 000-1: 5 000)
Verified Species Immunoglobulin Affinity  Human IgG1 (++++)
Rabbit IgG (+++)
Mouse IgG1 (+)
Mouse IgG2a (++++)
Alpaca IgG2b (++++)
Concentration 1.0 mg/ml
Form Liquid
Colour Light reddish
Preparation Ready to use
Storage Short term (up to one week): 2 – 8 °C
Long term: Aliquot and store at – 20 °C
Store immediately. Aliquot and avoid multiple freeze thaw cycles.
Storage buffer 0.1  M Phosphate Buffered Saline, pH 7.4. Preservative: None
Purification notes This product was purified using Immobilised metal affinity chromatography (IMAC).
Purity ≥ 85 % as determined by SDS-PAGE
97.06% as determined by Mass Spectrometry
General notes If for any reason the product does not perform as specified, please contact our scientific support team for assistance by emailing sales@capebiologix.com.

PtX™ | Plant-Based Expression

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single-use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins are extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.

Visit our Technology page to learn more.

Important!

  • For Research Use Only.
  • Not for diagnostic or therapeutic use.
  • Not for resale without express authorization.
  • Trademark.

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