Rabbit Anti-CD20 (1F5)

This product is a full-length Rabbit IgG1 recombinant antibody specific to CD20. It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.

100 µg      |     Catalogue Number: CBT_A0052

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CD20 is a cell surface protein expressed predominantly on B cells, playing a crucial role in their development, activation, and differentiation. It is involved in regulating B cell signaling and is implicated in B cell-mediated immune responses. CD20 is an appealing target for research due to its selective expression on B cells, making it a potential therapeutic target for B cell malignancies and autoimmune disorders.

Our PtX™ Rabbit Anti-CD20 (1F5) antibody enables researchers to investigate the distribution, activation, and maturation of B cells in different physiological and pathological contexts. This antibody is rigorously validated to ensure high specificity, sensitivity, and reproducibility, and is suitable for ELISA and western blot applications.  PtX™ Rabbit Anti-CD20 is an ideal tool for investigating the role of CD20 in disease pathogenesis and for developing novel therapeutic strategies targeting this protein.

Additional information

Available Unit Size

0.1mg, 1mg, 10mg, 100mg, 200mg

Key Benefits

This product is a recombinant monoclonal antibody that offers several benefits including the following:

  • Enhanced signal intensity and sensitivity
  • Reduced background noise
  • Compatibility with multiple species and antibody isotypes
  • Minimized steric hindrance
  • Batch-to-batch consistency.
  • No animals or animal products are used in the development of this protein.
  • BSA and Azide free.

For more information on plant-made, recombinant monoclonal antibodies click here. 

Datasheet

Product Name PtX™ Rabbit Anti-CD20 (1F5) Recombinant Antibody
Catalogue Number CBT_A0052
Expression Host Nicotiana benthamiana plants
Clonality Monoclonal, recombinant
Species and Isotype Rabbit IgG1
Tag None
Reporter Protein None
Description This product is a full-length Mouse IgG2a recombinant antibody specific to Human B-lymphocyte antigen CD20. It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.
Target Name Human B-lymphocyte antigen (CD20)
Verified Applications ELISA, Western blot
Dilution Range ELISA (1: 1 000 – 1: 5 000)

Western blot (1: 500 – 1: 1 000)
Tested Species Reactivity     Human
Concentration                   1 mg/ml
Form Liquid
Colour Clear
Preparation Ready to use
Storage Short term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze thaw cycles.
Storage Buffer 0.1M Phosphate Buffered Saline, pH 7.4
Purification Notes This product was purified using Protein A- affinity chromatography.
Purity ≥ 95 % as determined by SDS-PAGE
General Notes If for any reason the product does not perform as specified, please contact our scientific support team for assistance by emailing techsupport@capebiologix.com.

For Research Use only, unless otherwise indicated.

Images

Figure 1. ELISA Dose Response curve: using PtX™ Rabbit Anti-CD20 (1F5) Antibody from 20-0,0026 ng/uL to detect 50 ng Human B-lymphocyte antigen CD20 antigen. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot of decreasing amounts of Human CD20-His-Avi Tag (~16.8 kDa) detected with PtX™ Rabbit Anti-CD20 (1F5) at 1: 1 000 and an HRP conjugated anti-Rabbit secondary antibody at 1:10 000.

PtX™ | Plant-Based Expression

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single-use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.

Visit our Technology page to learn more.

Important!

  • For Research Use Only.
  • Not for diagnostic or therapeutic use.
  • Not for resale without express authorization.
  • Trademark.

Feel free to contact our trusted distribution partners directly if you want to order this product.

 

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