Human Anti-N (HuN4)

Recombinant human monoclonal antibody against SARS-CoV-2 Nucleocapsid protein. This product is produced in Nicotiana benthamiana plants for use in Western blot and ELISA applications.

100 µg      |     Catalogue Number: CBT_A0010

Download: SDS / Datasheet

View Product

Contact us

Share:

The SARS-CoV-2 Nucleocapsid (N) protein is the most abundantly expressed protein of the coronavirus. The N protein is composed of two protein domains connected by an intrinsically disordered region (IDR), known as the linker region. It binds to RNA to form helical ribonucleoprotein (RNP) structures during packaging of the genome into the viral capsid, while also regulating RNA synthesis during replication and transcription. N protein, along with the coronavirus membrane protein (M), facilitates viral assembly, while also modulating host cell metabolism and manipulating the cell cycle. Due to the strong immunogenicity and conserved nature of the coronavirus nucleocapsid, N proteins have value in diagnostic and vaccine development.

Additional information

Available Unit Size

0.1mg, 1mg, 10mg, 100mg, 200mg

Key Benefits

This product is a recombinant monoclonal antibody that offers several benefits including the following:

  • Batch-to-batch consistency.
  • No animals or animal products are used in the development of this protein.
  • BSA and Azide free.

For more information on plant-made, recombinant monoclonal antibodies click here.

Datasheet

Product Name PtX™ Human Anti-SARS CoV-2 Nucleocapsid Protein (HuN4) Recombinant Antibody
Catalogue Number CBT_A0010
Expression Host Nicotiana benthamiana plants
Clonality Monoclonal
Species and Isotype Human IgG1
Tag None
Reporter Protein None
Description Recombinant human monoclonal antibody against SARS-CoV-2 Nucleocapsid protein, produced via Agrobacterium tumefaciens infiltration of Nicotiana benthamiana plants.
Verified Applications Western blot, ELISA
Recommended Dilutions Western blot (1: 500 – 1: 2 000)
ELISA (1: 1 000 – 1: 4 000)
Concentration 1.0 mg/ml
Form Liquid
Colour Clear
Preparation Ready to use
Storage Short-term (up to one week): 2 – 8 °C
Long term: Aliquot and store at – 20 °C
Store immediately. Aliquot and avoid multiple freeze-thaw cycles.
Storage buffer 0.1  M Phosphate Buffered Saline, pH 7.4.
Preservative: none
Purification notes This product was purified using Protein A affinity chromatography.
Purity ≥ 95 % as determined by SDS-PAGE.
99.30% as determined by Mass Spectrometry.
General notes If for any reason the product does not perform as specified, please contact our scientific support team for assistance by emailing sales@capebiologix.com.

Images

Figure 1. ELISA Dose Response curve showing increasing absorbance at 370 nm with increasing amounts of PtX™ Human Anti-SARS-CoV-2 N (HuN4) Antibody, used to detect 1 ng/µl of SARS-CoV-2 Nucleocapsid Phosphoprotein.

Figure 2. Western blot analysis of PtX™ Human Anti-SARS-CoV-2 N (HuN4). Lanes 2 – 5: Varying amounts of SARS-CoV-2 Nucleocapsid (N) Phosphoprotein were run on the SDS-PAGE. Separated bands were transferred to the membrane and PtX™ Human Anti-N (HuN4) (1: 1 000) was used to detect the antigen. The SARS-CoV-2 N Phosphoprotein bands (~46 kDa) were visualized in each lane following the addition of anti-human secondary antibody with HRP and substrate. Our antibody was able to detect antigen amounts upwards of 15.6 ng (Lane 5).

PtX™ | Plant-Based Expression

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single-use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.

Visit our Technology page to learn more.

Important!

  • For Research Use Only.
  • Not for diagnostic or therapeutic use.
  • Not for resale without express authorization.
  • Trademark.

Feel free to contact our trusted distribution partners directly if you want to order this product.

 

Contact our Distribution Partners

Related products

  Sign up for our newsletter

Click Here