Rabbit Anti-c-Myc (9E10)

Recombinant rabbit monoclonal antibody against proto-oncogene; c-Myc and other c-Myc tagged proteins. This product is produced in Nicotiana benthamiana plants and is suitable for Western Blot and ELISA applications.

100 µg      |     Catalogue Number: CBT_A0007

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C-Myc is a 62 kDa transcription factor known for its role in cell division, differentiation, and apoptosis. Altered c-Myc expression is associated with tumor formation and is required for vasculogenesis and angiogenesis within tumor tissue. PtX™ Rabbit Anti-c-Myc (9E10), CBT_A0007, detects c-Myc protein, as well as c-Myc-tagged recombinant proteins, and has been validated for use in WB and ELISA.

Additional information

Available Unit Size

0.1mg, 1mg, 10mg, 100mg, 200mg

Key Benefits

This product is a plant-made, recombinant monoclonal antibody which offers several benefits including the following:

  • Capable of detecting c-Myc and recombinant proteins fused to c-Myc tags, upwards of 2.5ng.
  • No animals or animal products are used in the development of this protein.
  • BSA and Azide free.

For more information on plant-made, recombinant monoclonal antibodies click here.


Product Name PtX™ Rabbit Anti-c-Myc (9E10) Recombinant Antibody
Catalogue Number CBT_A0007
Expression Host Nicotiana benthamiana plants
Clonality Monoclonal, recombinant
Species and Isotype Rabbit IgG1
Tag None
Reporter Protein None
Description Recombinant rabbit monoclonal antibody against c-Myc proto-oncogene, produced via Agrobacterium tumefaciens infiltration of Nicotiana benthamiana plants.
Verified Applications Western blot (1: 1 000 – 1: 10 000)

ELISA (1: 1 000 – 1: 20 000)

Immunocytochemistry (ICC) (1: 100-1: 300)

Concentration 1.0 mg/ml
Form Liquid
Colour Clear
Preparation Ready to use
Storage Short-term (up to one week): 2 – 8 °C
Long term: Aliquot and store at – 20 °C
Store immediately. Aliquot and avoid multiple freeze-thaw cycles.
Storage buffer 0.1  M Phosphate Buffered Saline, pH 7.4.
Preservative: none
Purification notes This product was purified using Protein A affinity chromatography.
Purity ≥ 90 % as determined by SDS-PAGE.
97.24 % as determined by mass spectrometry
General notes If for any reason the product does not perform as specified, please contact our scientific support team for assistance by emailing techsupport@capebiologix.com.
For Research Use only, unless otherwise indicated.


Figure 1. ELISA Dose Response curve showing increasing absorbance at 370 nm with increasing amounts of PtX™ Rabbit Anti-c-Myc (9E10) antibody added to a 96 well plate coated with 0.1 ng/ µl c-Myc tagged Antigen.

Figure 2. Western blot analysis of PtX™ Rabbit Anti-c-Myc (9E10). Lanes 2 – 6: Varying amounts of c-Myc tagged Serglycin protein were run on the SDS-PAGE. Separated bands were transferred to the membrane and PtX™ Rabbit Anti-c-Myc (9E10) (1: 2 000) was used to detect the antigen. The single bands of c-Myc tagged protein were visualized in each lane following the addition of anti-rabbit secondary antibody with HRP and substrate. Our antibody was able to detect antigen amounts upwards of 2.5 ng (Lane 5).

Figure 3. Immunocytochemistry: HeLa cells were plated at 200 000 cells/ well in 6-well plates on coverslips and allowed to adhere. Following fixing and blocking, cells were incubated with 1:200 dilution of Cape Biologix PtX™ Rabbit Anti-c-Myc primary antibody (Cat# CBT_A0007), and 1:300 dilution of a commercial Anti-Rabbit Alexa Fluor® 488 conjugated commercial secondary antibody. Cells were then stained with Hoechst 33342. Images were taken on a Zeiss LSM780 with ELYRA PS1 platform confocal microscope (60X) at the Stellenbosch University CAF unit. Thank you to Prof Georgia Schafer (ICGEB) for kindly donating the HeLa cells and Mrs Lize Engelbrecht for her outstanding assistance.

PtX™ | Plant-Based Expression

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single-use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final

Visit our Technology page to learn more.


  • For Research Use Only.
  • Not for diagnostic or therapeutic use.
  • Not for resale without express authorization.
  • Trademark.

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