Rabbit Anti-BSA (Biotin)

Recombinant, biotinylated rabbit monoclonal antibody against Bovine Serum Albumin protein. This product is produced in Nicotiana benthamiana plants and is suitable for Western Blot and ELISA applications.

100 µg      |     Catalogue Number: CBT_A0014[B]

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Serum albumin is a major protein component of plasma with a molecular weight of 66kDa. It is synthesized in the liver as a non-glycosylated, negatively charged protein, capable of binding numerous lipophilic compounds, transporting fatty acids and regulating blood osmotic pressure. Our PtX™ Rabbit Anti-BSA (Biotin) binds bovine serum albumin and is suitable for use in Western blot and ELISA applications. The biotin labeling of this antibody ensures strong and stable interaction with avidin or streptavidin, making it suitable for a wide range of downstream applications. 

Additional information

Available Unit Size

0.1mg, 1mg, 10mg, 100mg, 200mg

Key Benefits

This product is a recombinant monoclonal antibody that offers several benefits including the following:

  • Enhanced signal intensity and sensitivity 
  • Reduced background noise 
  • Compatibility with multiple species and antibody isotypes 
  • Minimized steric hindrance 
  • Batch-to-batch consistency. 
  • No animals or animal products are used in the development of this protein. 
  • BSA and Azide free. 

For more information on plant-made, recombinant monoclonal antibodies click here.

Datasheet

Product Name PtX™ Rabbit Anti-Bovine Serum Albumin (Biotin) Recombinant Antibody
Catalogue Number CBT_A0014[B]
Expression Host Nicotiana benthamiana plants
Clonality Monoclonal
Species and Isotype Rabbit IgG1
Tag Biotin
Reporter Protein None
Description Recombinant, biotinylated rabbit monoclonal antibody against Bovine Serum Albumin protein, produced via Agrobacterium tumefaciens infiltration of Nicotiana benthamiana plants.
Verified Applications Western blot, ELISA
Species Reactivity Human, Bovine
Recommended Dilutions Western blot (1: 1 000 – 1: 5 000)
ELISA (1: 1 000– 1: 500 000)
Concentration 1.0 mg/ml
Form Liquid
Colour Clear
Preparation Ready to use
Storage Short-term (up to one week max): 2 – 8 °C
Long term: Aliquot and store at – 20 °C
Store immediately. Aliquot and avoid multiple freeze-thaw cycles.
Storage buffer 0.1 M Phosphate Buffered Saline, pH 7.4.
Preservative: none
Purification notes This product was purified using Protein A affinity chromatography.
Purity ≥ 95 % as determined by SDS-PAGE.
General notes If for any reason the product does not perform as specified, please contact our scientific support team for assistance by emailing sales@capebiologix.com.

Images

Figure 1. ELISA Dose Response curve showing PtX™ Rabbit Anti-BSA (Biotin) Antibody from 0.00016– 10.0 ng/uL used to detect 200 ng Bovine serum albumin (BSA) antigen. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot analysis showing decreasing amounts of Bovine serum albumin (BSA) (66 kDa) detected with PtX™ Rabbit Anti-BSA (Biotin) at 1: 1 000 and HRP conjugated Streptavidin at 1: 1000 dilution.

PtX™ | Plant-Based Expression

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single-use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.

Visit our Technology page to learn more.

Important!

  • For Research Use Only.
  • Not for diagnostic or therapeutic use.
  • Not for resale without express authorization.
  • Trademark.

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