Mouse Anti-CD47 (B6H12) (Biotin)

This product is a full-length biotinylated Mouse IgG2a recombinant antibody specific to Human CD47. It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.

100 µg      |     Catalogue Number: CBT_A0044[B]

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CD47, also known as integrin-associated protein (IAP), is a transmembrane protein that plays a crucial role in cell adhesion, migration, and signaling. Dysregulation of CD47 expression or function has been implicated in various diseases, including cancer, inflammatory disorders, and cardiovascular diseases. CD47 is frequently overexpressed on cancer cells and enables them to evade the immune system. Our anti-CD47 (B6H12) antibody is a high-affinity, monoclonal antibody that specifically recognizes and binds to CD47 and is suitable for use in Western blot, ELISA, ICC and flow cytometry. PtX™ Rabbit Anti-CD47 (B6H12) antibody is an ideal tool for investigating the role of CD47 in disease pathogenesis and for developing novel therapeutic strategies targeting this protein.

The biotin labelling of this antibody ensures strong and stable interaction with avidin or streptavidin, making it suitable for a wide range of downstream applications, including Western blotting and ELISA.

Additional information

Available Unit Size

0.1mg, 1mg, 10mg, 100mg, 200mg

Key Benefits

This product is a recombinant monoclonal antibody that offers several benefits including the following: 

  • Enhanced signal intensity and sensitivity
  • Reduced background noise
  • Compatibility with multiple species and antibody isotypes
  • Minimized steric hindrance.
  • Batch-to-batch consistency
  • This product is plant-made, no animals or animal products are used in the manufacture of this antibody.
  • BSA and Azide free

For more information on plant-made, recombinant monoclonal antibodies click here. 

Datasheet

Product Name PtX™ Mouse Anti-CD47 (Biotin) Recombinant Antibody
Catalogue Number CBT_A0044[B]
Expression Host Nicotiana benthamiana plants
Clonality Monoclonal, recombinant
Species and Isotype Mouse IgG2a
Tag Biotin
Reporter Protein None
Description This product is a full-length Mouse IgG2a recombinant antibody specific to Human CD47 (IAP) protein. It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.
Target Name Human CD47 (IAP) protein
Verified Applications Western blot, ELISA, Immunocytochemistry (ICC)
Dilution Range Western blot (1: 1 000 – 1: 10 000)

ELISA (1: 1 000 – 1: 250 000)

ICC (1:100-1:300)
Tested Species Reactivity     Human
Concentration                   1 mg/ml
Form Liquid
Colour Clear
Preparation Ready to use
Storage Short term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze thaw cycles.
Storage Buffer 0.1M Phosphate Buffered Saline, pH 7.4
Purification Notes This product was purified using Protein A- affinity chromatography.
Purity ≥ 95 % as determined by SDS-PAGE
General Notes If for any reason the product does not perform as specified, please contact our scientific support team for assistance by emailing techsupport@capebiologix.com.

For Research Use only, unless otherwise indicated.

Images

Figure 1. ELISA Dose Response curve: using PtX™ Mouse Anti-CD47 (Biotin) Antibody from 4.0 – 0,0000512 ng/uL to detect 25 ng Human CD47 protein antigen. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot of decreasing amounts of Human CD47 Protein (44 kDa) detected with PtX™ Mouse Anti-CD47 (Biotin) at 1: 5 000 and Streptavidin-HRP at 1: 5 000.

Figure 3. Immunocytochemistry: HeLa cells were plated at 200 000 cells/ well in 6-well plates on coverslips and allowed to adhere. Following fixing and blocking, cells were incubated with 1:200 dilution of Cape Biologix PtX™ Mouse Anti-CD47 (Biotin) primary antibody (Cat# CBT_A0044[B]), and 1:300 dilution of a commercial Anti-Mouse Alexa Fluor® 488 conjugated commercial secondary antibody. Images were taken on a Zeiss LSM780 with ELYRA PS1 platform confocal microscope (60X) at the Stellenbosch University CAF unit. Thank you to Prof Georgia Schafer (ICGEB) for kindly donating the HeLa cells and Mrs Lize Engelbrecht for her outstanding assistance.

PtX™ | Plant-Based Expression

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single-use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.

Visit our Technology page to learn more.

Important!

  • For Research Use Only.
  • Not for diagnostic or therapeutic use.
  • Not for resale without express authorization.
  • Trademark.

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