Human Anti-HA Tag(HRP)

This product is a full-length Human IgG1 recombinant antibody specific to Hemagglutinin Tag (HA Tag), fused at the genetic level to HRP reporter enzyme. It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.

100 µg      |     Catalogue Number: CBT_A0055

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HA tag is derived from amino acid residues 98 – 106 of the human influenza hemagglutinin (HA) molecule. Recombinant proteins are often engineered to express the HA tag as a general epitope tag in expression vectors. HA tag facilitates detection, isolation, and purification of target proteins without compromising its bioactivity or distribution. HA-tagging of a recombinant protein and subsequent examination using anti-HA antibody, in either intact or permeabilized cells, allows researchers to study cellular expression levels, topology and functional activity of the HA-tagged protein. Antibody mediated detection of the HA tagged proteins further negates the need for developing specific antibodies for newly identified proteins, which are often unstable, difficult to purify, cross-reactive or poorly immunogenic proteins.

Our PtX™ Human Anti-HA (HRP) antibody exhibits high specificity towards HA-tagged proteins and undergoes rigorous testing to ensure consistent performance and reliability.  Fusion at the genetic level to HRP allows for a single step, removing the need to add an HRP conjugated secondary antibody. This saves you time and money to focus on what really matters.  PtX™ Human Anti-HA (HRP) antibody is suitable for use in ELISA and Western blot applications.

Additional information

Available Unit Size

0.1mg, 1mg, 10mg, 100mg, 200mg

Key Benefits

This product is a recombinant monoclonal antibody that offers several benefits including the following: 

  • Enhanced reproducibility associated with HRP fusion
  • Reduced Background Noise
  • Time and Cost Efficiency
  • Compatibility with Chemiluminescent and Colorimetric Detection
  • High batch-to-batch consistency
  • No animals or animal products are used in the production of this antibody
  • BSA and Azide

For more information on plant-made, recombinant monoclonal antibodies click here.


Product Name PtX™ Human Anti-HA Tag (12CA5) (HRP)
Catalogue Number CBT_A0055
Expression Host Nicotiana benthamiana plants
Clonality Monoclonal, recombinant
Species and Isotype Human IgG1
Tag None
Reporter Protein Horseradish peroxidase (HRP)
Description This product is a full-length Human IgG1 recombinant antibody specific to Hemagglutinin Tag (HA Tag) and fused at the genetic level to HRP. It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.
Target Name Hemagglutinin Tag (HA Tag)
Verified Applications Western blot, ELISA
Dilution Range Western blot (1: 1 000 – 1: 2 000)

ELISA (1: 1 000 – 1: 160 000)

Tested Species Reactivity     Human
Concentration                   1 mg/ml
Form Liquid
Colour Clear with a faint red tinge
Preparation Ready to use
Storage Short term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze thaw cycles.

Storage Buffer 0.1M Phosphate Buffered Saline, pH 7.4
Preservative: 50% Glycerol
Purification Notes This product was purified using Protein A- affinity chromatography.
Purity 95 % as determined by SDS-PAGE
General Notes If for any reason the product does not perform as specified, please contact our scientific support team for assistance by emailing

For Research Use only, unless otherwise indicated.


Figure 1. ELISA Dose Response curve using PtX™ Human Anti-HA Tag (12CA5) (HRP) Antibody from 40– 0,008 ng/uL to detect 10 ng HA peptide antigen. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot: Decreasing amounts of HA-tagged mouse recombinant CD40 ligand (25 – 35 kDa) detected with PtX™ Human Anti-HA Tag (12CA5) (HRP) at 1: 1 000.

PtX™ | Plant-Based Expression

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single-use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.

Visit our Technology page to learn more.


  • For Research Use Only.
  • Not for diagnostic or therapeutic use.
  • Not for resale without express authorization.
  • Trademark.

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