Human Anti-HA Tag(HRP)

This product is a full-length Human IgG1 recombinant antibody specific to Hemagglutinin Tag (HA Tag), fused at the genetic level to HRP reporter enzyme. It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.

100 µg      |     Catalogue Number: CBT_A0055

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HA tag is derived from amino acid residues 98 – 106 of the human influenza hemagglutinin (HA) molecule. Recombinant proteins are often engineered to express the HA tag as a general epitope tag in expression vectors. HA tag facilitates detection, isolation, and purification of target proteins without compromising its bioactivity or distribution. HA-tagging of a recombinant protein and subsequent examination using anti-HA antibody, in either intact or permeabilized cells, allows researchers to study cellular expression levels, topology and functional activity of the HA-tagged protein. Antibody mediated detection of the HA tagged proteins further negates the need for developing specific antibodies for newly identified proteins, which are often unstable, difficult to purify, cross-reactive or poorly immunogenic proteins.

Our PtX™ Human Anti-HA (HRP) antibody exhibits high specificity towards HA-tagged proteins and undergoes rigorous testing to ensure consistent performance and reliability.  Fusion at the genetic level to HRP allows for a single step, removing the need to add an HRP conjugated secondary antibody. This saves you time and money to focus on what really matters.  PtX™ Human Anti-HA (HRP) antibody is suitable for use in ELISA and Western blot applications.

Additional information

Available Unit Size

0.1mg, 1mg, 10mg, 100mg, 200mg

Key Benefits

This product is a recombinant monoclonal antibody that offers several benefits including the following: 

  • Enhanced reproducibility associated with HRP fusion
  • Reduced Background Noise
  • Time and Cost Efficiency
  • Compatibility with Chemiluminescent and Colorimetric Detection
  • High batch-to-batch consistency
  • No animals or animal products are used in the production of this antibody
  • BSA and Azide

For more information on plant-made, recombinant monoclonal antibodies click here.

Datasheet

Product Name PtX™ Human Anti-HA Tag (12CA5) (HRP)
Catalogue Number CBT_A0055
Expression Host Nicotiana benthamiana plants
Clonality Monoclonal, recombinant
Species and Isotype Human IgG1
Tag None
Reporter Protein Horseradish peroxidase (HRP)
Description This product is a full-length Human IgG1 recombinant antibody specific to Hemagglutinin Tag (HA Tag) and fused at the genetic level to HRP. It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.
Target Name Hemagglutinin Tag (HA Tag)
Verified Applications Western blot, ELISA
Dilution Range Western blot (1: 1 000 – 1: 2 000)

ELISA (1: 1 000 – 1: 160 000)

Tested Species Reactivity     Human
Concentration                   1 mg/ml
Form Liquid
Colour Clear with a faint red tinge
Preparation Ready to use
Storage Short term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze thaw cycles.

Storage Buffer 0.1M Phosphate Buffered Saline, pH 7.4
Preservative: 50% Glycerol
Purification Notes This product was purified using Protein A- affinity chromatography.
Purity 95 % as determined by SDS-PAGE
General Notes If for any reason the product does not perform as specified, please contact our scientific support team for assistance by emailing techsupport@capebiologix.com.

For Research Use only, unless otherwise indicated.

Images

Figure 1. ELISA Dose Response curve using PtX™ Human Anti-HA Tag (12CA5) (HRP) Antibody from 40– 0,008 ng/uL to detect 10 ng HA peptide antigen. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot: Decreasing amounts of HA-tagged mouse recombinant CD40 ligand (25 – 35 kDa) detected with PtX™ Human Anti-HA Tag (12CA5) (HRP) at 1: 1 000.

PtX™ | Plant-Based Expression

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single-use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.

Visit our Technology page to learn more.

Important!

  • For Research Use Only.
  • Not for diagnostic or therapeutic use.
  • Not for resale without express authorization.
  • Trademark.

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