Rabbit Anti-Alpha Tubulin (F2C)

Recombinant rabbit monoclonal IgG1 antibody against Human Alpha Tubulin. This product is produced in Nicotiana benthamiana plants and is suitable for Western Blot and ELISA applications.

100 µg      |     Catalogue Number: CBT_A0057

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Alpha-tubulin is a globular protein which forms heterodimers with beta-tubulin to produce microtubules, the cytoskeletal filaments found in eukaryotic cells. The amino acid sequence of alpha-tubulin is highly conserved across eukaryotes, and contains a GTP-binding domain, which is important for regulating the assembly and disassembly of microtubules. Alpha-tubulin plays a crucial role in cell division, intracellular transport, and the maintenance of cell shape and polarity. Mutations in the alpha-tubulin gene have been linked to various diseases, including developmental disorders and cancer. The study of alpha-tubulin and its interactions with other proteins has important implications for understanding the basic biology of cells and the development of new therapies for human diseases.

Additional information

Available Unit Size

0.1mg, 1mg, 10mg, 100mg, 200mg

Key Benefits

This product is a recombinant monoclonal antibody that offers several benefits including the following:

  • Batch-to-batch consistency.
  • This product is plant-made, no animals or animal products are used in the manufacture of this protein.
  • BSA and Azide free.

For more information on plant-made, recombinant monoclonal antibodies click here.

Datasheet

Product Name PtX™ Rabbit Anti-Alpha Tubulin (F2C) Recombinant Antibody
Catalogue Number CBT_A0057
Expression Host Nicotiana benthamiana plants
Clonality Monoclonal, recombinant
Species and Isotype Rabbit IgG1
Tag None
Reporter Protein None
Description This product is a full-length Rabbit IgG1 recombinant antibody specific to Alpha Tubulin. It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.
Target Name Alpha Tubulin
Verified Applications Western blot, ELISA, Immunocytochemistry
Dilution Range Western blot (1: 1 000 – 1: 5 000)

ELISA (1: 1 000 – 1: 160 000)

ICC (1: 100-1: 400)
Tested Species Reactivity     Human, Mouse
Concentration                   1.0 mg/ml
Form Liquid
Colour Clear
Preparation Ready to use
Storage Short term (up to one week): 2 – 8 °C. Long term: Aliquot and store at – 20 °C.  Store immediately. Aliquot and avoid multiple freeze thaw cycles.
Storage Buffer 0.1   M Phosphate Buffered Saline, pH 7.4. Preservative: None
Purification Notes This product was purified using Protein A- affinity chromatography.
Purity 95 % as determined by SDS-PAGE
General Notes If for any reason the product does not perform as specified, please contact our scientific support team for assistance by emailing techsupport@capebiologix.com.

For Research Use only, unless otherwise indicated.

Images

Figure 1. ELISA Dose Response curve using PtX™ Rabbit Anti-Alpha Tubulin Antibody from 0,0002– 8,0 ng/uL to detect 25 ng Alpha 1a Tubulin, His Tagged antigen. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot of decreasing amounts of HeLa Cell Lysate containing Alpha Tubulin (50 kDa) detected with PtX™ Rabbit Anti-Alpha Tubulin (F2C) at 1: 5 000 and an HRP conjugated anti-Rabbit secondary antibody at 1:30 000.

Figure 3. Immunocytochemistry: HeLa cells were plated at 200 000 cells/ well in 6-well plates on coverslips and allowed to adhere. Following fixation and blocking, cells were incubated with 1:200 dilution of Cape Biologix PtX™ Rabbit Anti-Alpha-Tubulin primary antibody (Cat# CBT_A0057), and 1:300 dilution of a commercial Anti-Rabbit Alexa Fluor® 488 conjugated commercial secondary antibody. Images were taken on a Zeiss LSM780 with ELYRA PS1 platform confocal microscope (20X) at the Stellenbosch University CAF unit. Thank you to Prof Georgia Schafer (ICGEB) for kindly donating the HeLa cells and Mrs Lize Engelbrecht for her outstanding assistance.

PtX™ | Plant-Based Expression

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single-use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.

Visit our Technology page to learn more.

Important!

  • For Research Use Only.
  • Not for diagnostic or therapeutic use.
  • Not for resale without express authorization.
  • Trademark.

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