Rabbit Anti-Beta Tubulin

Recombinant rabbit monoclonal antibody against Beta Tubulin. This product is produced in Nicotiana benthamiana plants for use in Western blot application.

100 µg Sample Size     |     Catalogue Number: CBT_A0002

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Tubulin is a major component of microtubules, the scaffolding of the eukaryotic cytoskeleton. It plays multiple roles in the maintenance of cell structure, intracellular vesicle and organelle transport, and cell division (mitosis and meiosis). Microtubules are formed by polymerization of alpha and beta tubulin isotypes, and the addition of proteins referred to as microtubule-associated proteins (MAPs). It has a molecular weight of 50kDa and is composed of a double β-sheet core, enclosed within α-helices. Beta tubulin is a highly conserved, negatively charged protein, which has made it a popular target for anti-cancer drugs, aimed at disrupting microtubule dynamics and cell division. Anti-beta tubulin antibodies are commonly used as loading controls for immunoblotting to verify that uniform quantities of protein have been loaded across wells.

Additional information

Available Unit Size

0.1mg, 1mg, 10mg, 100mg, 200mg

Key Benefits

This product is a plant-made, recombinant monoclonal antibody, which offers several advantages including:

  • Compatible with our PtX™ Protein A-HRP (CBT_P0002) detection protein.
  • Protein is fused at the genetic level for increased reproducibility of results.
  • No animals or animal products are used in the development of this protein.
  • BSA and Azide free.

For more information on plant-made, recombinant monoclonal antibodies click here.

Datasheet

Product Name PtX™ Rabbit Anti-Beta Tubulin (S11B) Recombinant Antibody
Catalogue Number CBT_A0002
Expression Host Nicotiana benthamiana plants
Clonality Monoclonal, recombinant
Species and Isotype Rabbit IgG1
Tag None
Reporter Protein None
Description This product is a full-length Rabbit IgG1 recombinant antibody specific to Beta Tubulin. It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.
Target Name Beta Tubulin
Verified Applications Western blot, ELISA
Dilution Range Western blot (1: 1 000 – 1: 5 000). ELISA (end user to determine)
Tested Species Reactivity     Human, Mouse
Concentration                   1 mg/ml
Form Liquid
Colour Clear to light yellow
Preparation Ready to use
Storage Short term (up to one week): 2 – 8 °C. Long term: Aliquot and store at – 20 °C. Store immediately. Aliquot and avoid multiple freeze thaw cycles.
Storage Buffer 0.1   M Phosphate Buffered Saline, pH 7.4. Preservative: None.
Purification Notes This product was purified using Protein A- affinity chromatography.
Purity 95 % as determined by SDS-PAGE. 97.67% as determined by Mass Spectrometry
General Notes If for any reason the product does not perform as specified, please contact our scientific support team for assistance by emailing techsupport@capebiologix.com.

For Research Use only, unless otherwise indicated.

Images

Figure 1. Western blot images of PtX™ Rabbit Anti-Beta Tubulin used at 1: 5 000 to detect Beta tubulin protein (50 kDa) within cell lysates. A) 1 µg of various cell lysates B) Decreasing amounts (1 000 ng-62.5 ng) of HeLa cell lysates, and C) Decreasing amounts (100 ng-6.25 ng) of commercial recombinant Beta tubulin protein.

PtX™ | Plant-Based Expression

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.

Visit our Technology page to learn more.

Important!

  • For Research Use Only.
  • Not for diagnostic or therapeutic use.
  • Not for resale without express authorization.
  • Trademark.

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