Mouse Anti-VEGF (Biotin)

This product is a full-length Biotinylated Mouse IgG2a recombinant antibody specific to Vascular Endothelial Growth Factor (VEGF-165a). It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.

100 µg      |     Catalogue Number: CBT_A0019[B]

Download: SDS / Datasheet

View Product

Contact us

Share:

Vascular endothelial growth factor (VEGF) is a signalling protein that plays a crucial role in angiogenesis, the formation of new blood vessels from pre-existing ones. It is produced by many different cell types, including endothelial cells, macrophages, and tumour cells, and is a key regulator of blood vessel growth and maintenance. The physiological functions of VEGF include promoting the proliferation and migration of endothelial cells, as well as increasing vascular permeability. VEGF also stimulates the production of nitric oxide, a potent vasodilator, which helps to increase blood flow to tissues. In addition to its role in normal tissue growth and repair, VEGF is also involved in pathological processes such as tumour angiogenesis and ocular neovascularization. VEGF has been found to be overexpressed in many types of cancer, including breast, lung, colorectal, and kidney cancer, among others. Its overexpression has been associated with increased tumour growth, invasion, and metastasis, as well as poor prognosis and resistance to chemotherapy and radiotherapy.

The biotin labelling of this antibody ensures strong and stable interaction with avidin or streptavidin, making it suitable for a wide range of downstream applications.

Additional information

Available Unit Size

0.1mg, 1mg, 10mg, 100mg, 200mg

Key Benefits

This product is a recombinant monoclonal antibody that offers several benefits including the following:

  • Enhanced signal intensity and sensitivity
  • Reduced background noise
  • Compatibility with multiple species and antibody isotypes
  • Minimized steric hindrance.
  • Batch-to-batch consistency.
  • No animals or animal products are used in the development of this protein.
  • BSA and Azide free.

For more information on plant-made, recombinant monoclonal antibodies click here. 

Datasheet

Product Name PtX™ Mouse Anti-Vascular Endothelial Growth Factor (VEGF) (Biotin) Recombinant Antibody
Catalogue Number CBT_A0019[B]
Expression Host Nicotiana benthamiana plants
Clonality Monoclonal, recombinant
Species and Isotype Mouse IgG2a
Tag Biotin
Reporter Protein None
Description This product is a full-length Mouse IgG2a recombinant antibody specific to Vascular Endothelial Growth Factor (VEGF-165a). It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.
Target Name Vascular Endothelial Growth Factor (VEGF-165a)
Verified Applications ELISA, Western blot
Dilution Range ELISA (1: 1 000 – 1: 10 000)

Western blot (1: 1 000 – 1: 2 000)
Tested Species Reactivity     Human
Concentration                   1 mg/ml
Form Liquid
Colour Clear
Preparation Ready to use
Storage Short term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze thaw cycles.
Storage Buffer 0.1M Phosphate Buffered Saline, pH 7.4
Purification Notes This product was purified using Protein A- affinity chromatography.
Purity ≥ 95 % as determined by SDS-PAGE
General Notes If for any reason the product does not perform as specified, please contact our scientific support team for assistance by emailing techsupport@capebiologix.com.

For Research Use only, unless otherwise indicated.

Images

Figure 1. ELISA Dose Response curve using PtX™ Mouse Anti-VEGF (Biotin) Antibody from 4.0– 0.00005 ng/uL to detect 50 ng Recombinant Human VEGF antigen. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot of decreasing amounts of Recombinant Human VEGF (~28 kDa) detected with PtX™ Mouse Anti-VEGF (Biotin) at 1: 1 000 and HRP conjugated Streptavidin at 1: 5 000.

PtX™ | Plant-Based Expression

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single-use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.

Visit our Technology page to learn more.

Important!

  • For Research Use Only.
  • Not for diagnostic or therapeutic use.
  • Not for resale without express authorization.
  • Trademark.

Feel free to contact our trusted distribution partners directly if you want to order this product.

 

Contact our Distribution Partners

Related products

  Sign up for our newsletter

Click Here