Mouse Anti-IL-8

Recombinant mouse monoclonal antibody against Interleukin-8. This product is produced in Nicotiana benthamiana plants and is suitable for Western Blot and ELISA applications.

100 µg      |     Catalogue Number: CBT_A0033

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Interleukin-8 (IL-8, CXCL8) is a pro-inflammatory cytokine that is a member of the chemokine family. Cytokines are small signalling molecules that are secreted by immune cells and other cell types in response to infection, injury, or other stimuli. IL-8 is produced by a variety of cell types, including immune cells, endothelial cells, and epithelial cells, and plays an important role in the immune response to infection and inflammation. IL-8 binds to specific receptors on the surface of neutrophils, called CXCR1 and CXCR2, which activate signalling pathways within the cells that lead to their migration towards the site of infection or inflammation. In addition, IL-8 also activate other immune cells such as T cells and natural killer cells and promote angiogenesis in tissues undergoing repair or remodelling. Excessive or chronic production of IL-8 can contribute to the pathogenesis of a variety of inflammatory and autoimmune diseases, such as rheumatoid arthritis, chronic obstructive pulmonary disease (COPD), and cancer.

Additional information

Available Unit Size

0.1mg, 1mg, 10mg, 100mg, 200mg

Key Benefits

This product is a recombinant monoclonal antibody that offers several benefits including the following:

  • Batch-to-batch consistency.
  • This product is plant-made, no animals or animal products are used in the manufacture of this protein.
  • BSA and Azide free.

For more information on plant-made, recombinant monoclonal antibodies click here.

Datasheet

Product Name PtX™ Mouse Anti-Interleukin-8 Recombinant Antibody
Catalogue Number CBT_A0033
Expression Host Nicotiana benthamiana plants
Clonality Monoclonal, recombinant
Species and Isotype Mouse IgG2a
Tag None
Reporter Protein None
Description This product is a full-length Mouse IgG2a recombinant antibody specific to Interleukin 8 (also known as CXCL8) Antigen. It was produced in Nicotiana benthamiana plants via
Agrobacterium tumefaciens mediated infiltration.
Target name Interleukin 8 (CXCL8)
Verified Applications Western blot, ELISA
Dilution Range WB (1: 5 000 – 1: 10 000
ELISA (1: 30 000 – 1: 1 000 000)
Concentration 1.0 mg/ml
Form Liquid
Colour Clear
Preparation Ready to use
Storage Short term (up to one week): 2 – 8 °C
Long term: Aliquot and store at – 20 °C
Store immediately. Aliquot and avoid multiple freeze thaw cycles.
Storage Buffer 0.1  M Phosphate Buffered Saline, pH 7.4
Preservative: None
Purification Notes This product was purified using Protein A affinity chromatography.
Purity ≥ 90 % as determined by SDS-PAGE
General Notes If for any reason the product does not perform as specified, please contact our scientific support team for assistance by emailing sales@capebiologix.com.

Images

Figure 1. ELISA Dose Response curve using PtX™ Mouse Anti-IL-8 to detect coated IL-8 Antigen at 0,25 ng/µl and increasing concentrations of antibody. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Functionality western blot analysis using PtX™ Mouse Anti-IL-8 (1: 10 000) to detect decreasing amounts of IL-8 Antigen (~12 kDa) and detected using an Anti-Mouse secondary conjugated to HRP at 1: 10 000.

Figure 3. ELISA Dose Response curve using PtX™ Mouse Anti-IL-8 to detect coated IL-8 Antigen stored at -20ᵒC and shipping conditions for 2 weeks at 1 µg/µl. At shipping the product is placed under conditions that simulate real time shipping conditions. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

 

PtX™ | Plant-Based Expression

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single-use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.

Visit our Technology page to learn more.

Important!

  • For Research Use Only.
  • Not for diagnostic or therapeutic use.
  • Not for resale without express authorization.
  • Trademark.

Feel free to contact our trusted distribution partners directly if you want to order this product.

 

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