Human Anti-His tag(HRP)

Horseradish peroxidase (HRP) conjugated recombinant monoclonal antibody against C-terminal polyhistidine tagged proteins. Produced in Nicotiana benthamiana plants and suitable for Western blot and ELISA applications.

100 µg      |     Catalogue Number: CBT_A0022

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Polyhistidine tag, commonly referred to as his-tag, is a synthetic oligopeptide consisting of 6 - 10 consecutive histidine residues inserted into the amino acid sequence of recombinant proteins. His-tags are genetically fused to target proteins, creating a cluster of histidine residues at either the N- or C-terminal region of the protein. Histidine residues have strong and selective binding affinity to metal ions, e.g. Ni2+, making His-tagging the preferred method for recombinant protein purification. His-tag is also used in conjunction with anti-His antibodies for detection of proteins for which no antibody exists. PtX™ Human Anti-His tag (C-term) (HRP) is designed to specifically recognize and bind C-terminal His-tagged proteins, making it ideal for Western blotting and ELISA applications. This antibody is genetically fused to HRP, a highly sensitive enzyme that produces a strong signal in detection assays, allowing for the easy detection and quantification of His-tagged proteins.

Additional information

Available Unit Size

0.1mg, 1mg, 10mg, 100mg, 200mg

Key Benefits

This product is a recombinant monoclonal antibody that offers several benefits including the following:

  • Genetically fused to HRP to provide a convenient, ready to use format.
  • Able to detect polyhistidine tags.
  • Batch-to-batch consistency.
  • No animals or animal products are used in the development of this protein.
  • BSA and Azide free.

For more information on plant-made, recombinant monoclonal antibodies click here.

Datasheet

Product Name PtX™ Human Anti-His tag (C-term) (3D5) Recombinant Antibody (HRP)
Catalogue Number CBT_A0022
Expression Host Nicotiana benthamiana plants
Clonality Monoclonal, recombinant
Species and Isotype Human IgG1
Tag None
Reporter Protein None
Description This product is a full-length Human IgG1 antibody specific to Polyhistidine tag/ purified his-tagged Fab fragment at the carboxy-terminal molecule. It was produced recombinantly in Nicotiana benthamiana plants via Agrobacterium tumefaciens mediated infiltration.
Target Name Polyhistidine tag/ purified his-tagged Fab fragment at the carboxy-terminal
Verified Applications Western blot, ELISA
Recommended Dilutions Western blot (1: 500 -1: 5 000)
ELISA (1: 1 000 – 1: 150 000)
Concentration 0.5 mg/ml
Form Liquid
Colour Clear to light yellow
Preparation Ready to use
Storage Short-term (up to one week): 2 – 8 °C
Long term: Aliquot and store at – 20 °C
Store immediately. Aliquot and avoid multiple freeze-thaw cycles.
Storage Buffer 0.1  M Phosphate Buffered Saline, pH 7.4
Preservative: 25% Glycerol
Purification Notes This product was purified using Protein A affinity chromatography.
Purity ≥ 95% as determined by SDS-PAGE
General notes If for any reason the product does not perform as specified, please contact our scientific support team for assistance by emailing sales@capebiologix.com.

Images

Figure 1. ELISA Dose Response curve using PtX™ Human Anti-His tag (C-term) (3D5) (HRP) to detect coated S1-His Antigen at 1 ng/µl and increasing concentrations of antibody. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot of decreasing amounts of S1-His antigen (73 kDa) detected using PX™ Human Anti-His tag (C-term) (3D5) (HRP) at 1: 5 000.

PtX™ | Plant-Based Expression

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single-use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.

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Important!

  • For Research Use Only.
  • Not for diagnostic or therapeutic use.
  • Not for resale without express authorization.
  • Trademark.

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