Human Anti-His tag(HRP)

Horseradish peroxidase (HRP) conjugated recombinant monoclonal antibody against C-terminal polyhistidine tagged proteins. Produced in Nicotiana benthamiana plants and suitable for Western blot and ELISA applications.

100 µg      |     Catalogue Number: CBT_A0022

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Polyhistidine tag, commonly referred to as his-tag, is a synthetic oligopeptide consisting of 6 - 10 consecutive histidine residues inserted into the amino acid sequence of recombinant proteins. His-tags are genetically fused to target proteins, creating a cluster of histidine residues at either the N- or C-terminal region of the protein. Histidine residues have strong and selective binding affinity to metal ions, e.g. Ni2+, making His-tagging the preferred method for recombinant protein purification. His-tag is also used in conjunction with anti-His antibodies for detection of proteins for which no antibody exists. PtX™ Human Anti-His tag (C-term) (HRP) is designed to specifically recognize and bind C-terminal His-tagged proteins, making it ideal for Western blotting and ELISA applications. This antibody is genetically fused to HRP, a highly sensitive enzyme that produces a strong signal in detection assays, allowing for the easy detection and quantification of His-tagged proteins.

Additional information

Available Unit Size

0.1mg, 1mg, 10mg, 100mg, 200mg

Key Benefits

This product is a recombinant monoclonal antibody that offers several benefits including the following:

  • Genetically fused to HRP to provide a convenient, ready to use format.
  • Able to detect polyhistidine tags.
  • Batch-to-batch consistency.
  • No animals or animal products are used in the development of this protein.
  • BSA and Azide free.

For more information on plant-made, recombinant monoclonal antibodies click here.


Product Name PtX™ Human Anti-His tag (C-term) (3D5) Recombinant Antibody (HRP)
Catalogue Number CBT_A0022
Expression Host Nicotiana benthamiana plants
Clonality Monoclonal, recombinant
Species and Isotype Human IgG1
Tag None
Reporter Protein None
Description This product is a full-length Human IgG1 antibody specific to Polyhistidine tag/ purified his-tagged Fab fragment at the carboxy-terminal molecule. It was produced recombinantly in Nicotiana benthamiana plants via Agrobacterium tumefaciens mediated infiltration.
Target Name Polyhistidine tag/ purified his-tagged Fab fragment at the carboxy-terminal
Verified Applications Western blot, ELISA
Recommended Dilutions Western blot (1: 500 -1: 5 000)
ELISA (1: 1 000 – 1: 150 000)
Concentration 0.5 mg/ml
Form Liquid
Colour Clear to light yellow
Preparation Ready to use
Storage Short-term (up to one week): 2 – 8 °C
Long term: Aliquot and store at – 20 °C
Store immediately. Aliquot and avoid multiple freeze-thaw cycles.
Storage Buffer 0.1  M Phosphate Buffered Saline, pH 7.4
Preservative: 25% Glycerol
Purification Notes This product was purified using Protein A affinity chromatography.
Purity ≥ 95% as determined by SDS-PAGE
General notes If for any reason the product does not perform as specified, please contact our scientific support team for assistance by emailing


Figure 1. ELISA Dose Response curve using PtX Human Anti-His tag (C-term) (3D5) (HRP) to detect coated S1-His Antigen at 1 ng/µl and increasing concentrations of antibody. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot of decreasing amounts of S1-His antigen (73 kDa) detected using PX Human Anti-His tag (C-term) (3D5) (HRP) at 1: 5 000.

PtX™ | Plant-Based Expression

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single-use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.

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  • For Research Use Only.
  • Not for diagnostic or therapeutic use.
  • Not for resale without express authorization.
  • Trademark.

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