Mouse Anti-HA Tag (Biotin)

This product is a full-length biotinylated Mouse IgG2a recombinant antibody specific to HA Tag. It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.

100 µg      |     Catalogue Number: CBT_A0050[B]

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The HA tag is derived from amino acid residues 98 – 106 of the human influenza hemagglutinin (HA) molecule. Recombinant proteins are often engineered to express the HA tag as a general epitope tag in expression vectors. HA tag facilitates detection, isolation, and purification of target proteins without compromising its bioactivity or distribution. HA-tagging of a recombinant protein and subsequent examination using anti-HA antibody, in either intact or permeabilized cells, allows researchers to study cellular expression levels, topology and functional activity of the HA-tagged protein. Antibody mediated detection of the HA tagged proteins further negates the need for developing specific antibodies for newly identified proteins, which are often unstable, difficult to purify, cross-reactive or poorly immunogenic proteins. Our PtX™ Mouse Anti-HA antibody exhibits high specificity towards HA-tagged proteins and undergoes rigorous testing to ensure consistent performance and reliability. The biotin labelling of this antibody ensures strong and stable interaction with avidin or streptavidin, making it suitable for a wide range of downstream applications, including Western blotting and ELISA.

Additional information

Available Unit Size

0.1mg, 1mg, 10mg, 100mg, 200mg

Key Benefits

This product is a recombinant monoclonal antibody that offers several benefits including the following:

  • Batch-to-batch consistency.
  • No animals or animal products are used in the development of this protein.
  • BSA and Azide free.

For more information on plant-made, recombinant monoclonal antibodies click here.

Datasheet

Product Name PtX™ Mouse Anti-HA Tag (12CA5) (Biotin) Recombinant Antibody
Catalogue Number CBT_A0050[B]
Expression Host Nicotiana benthamiana plants
Clonality Monoclonal, recombinant
Species and Isotype Mouse IgG2a
Tag Biotin
Reporter Protein None
Description This product is a full-length biotinylated Mouse IgG2a recombinant antibody specific to the HA Tag. It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.
Target Name Human influenza hemagglutinin (HA) Tag
Verified Applications Western blot, ELISA, fluorescent western blot
Dilution Range Western blot (1: 1 000 – 1: 2 000)

ELISA (1: 1 000 – 1: 10 000)
Tested Species Reactivity     Human
Concentration                   1.0 mg/ml
Form Liquid
Colour Clear
Preparation Ready to use
Storage Short term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze thaw cycles.
Storage Buffer 0.1M Phosphate Buffered Saline, pH 7.4
Purification Notes This product was purified using Protein A- affinity chromatography.
Purity 95 % as determined by SDS-PAGE
General Notes If for any reason the product does not perform as specified, please contact our scientific support team for assistance by emailing techsupport@capebiologix.com.

For Research Use only, unless otherwise indicated.

Images

Figure 1. ELISA Dose Response curve: PtX™ Mouse Anti-HA Tag (Biotin) Antibody from 0.00024– 4.0 ng/uL used to detect 25 ng HA Peptide. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot: decreasing amounts of HA-tagged mouse recombinant CD40 ligand (25 – 35 kDa) detected with PtX™ Mouse Anti-HA Tag (Biotin) at 1: 1 000 and an HRP conjugated streptavidin secondary antibody.

 

PtX™ | Plant-Based Expression

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single-use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.

Visit our Technology page to learn more.

Important!

  • For Research Use Only.
  • Not for diagnostic or therapeutic use.
  • Not for resale without express authorization.
  • Trademark.

Feel free to contact our trusted distribution partners directly if you want to order this product.

 

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