Mouse Anti-Delta-9-THC (Biotin)

This product is a full-length biotinylated Mouse IgG2a recombinant antibody specific to Delta-9-THC. It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.

100 µg      |     Catalogue Number: CBT_A0015[B]

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Delta-9-tetrahydrocannabinol is a psychoactive compound found in the cannabis plant. It is the primary active ingredient responsible for the plant's euphoric effects and is known for its ability to alter mood, perception, and cognition. Delta-9-THC works by binding cannabinoid receptors in the brain and nervous system, mimicking the effects of naturally occurring neurotransmitters. It has been shown to have both therapeutic and recreational uses, with research suggesting its potential in treating conditions such as chronic pain, nausea, and anxiety. TX™ Mouse Anti-Delta-9-THC is a high-affinity monoclonal antibody that is engineered to detect THC in a variety of matrices, including biological fluids and plant extracts. The biotin labelling of this antibody ensures strong and stable interaction with avidin or streptavidin, making it suitable for a range of downstream applications, including Western blotting and ELISA and flow cytometry.

The biotin labelling of this antibody ensures strong and stable interaction with avidin or streptavidin, making it suitable for a wide range of downstream applications, including Western blotting and ELISA.

Additional information

Available Unit Size

0.1mg, 1mg, 10mg, 100mg, 200mg

Key Benefits

This product is a recombinant monoclonal antibody that offers several benefits including the following:

  • Enhanced signal intensity and sensitivity
  • Reduced background noise
  • Compatibility with multiple species and antibody isotypes
  • Minimized steric hindrance
  • Batch-to-batch consistency.
  • This product is plant-made, no animals or animal products are used in the manufacture of this protein.
  • BSA and Azide free.

For more information on plant-made, recombinant monoclonal antibodies click here.

Datasheet

Product Name PtX™ Mouse Anti-Delta-9-THC (Biotin) Recombinant Antibody
Catalogue Number CBT_A0015[B]
Expression Host Nicotiana benthamiana plants
Clonality Monoclonal, recombinant
Species and Isotype Mouse IgG2a
Tag Biotin
Reporter Protein None
Description This product is a full-length Mouse IgG2a recombinant antibody specific to Delta-9-THC. It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.
Target Name Delta-9-Tetrahydrocannabinol (THC)
Verified Applications Western blot, ELISA
Dilution Range Western blot (1: 1 000 – 1: 5 000)

ELISA (1: 1 000 – 1: 65 000)

Tested Species Reactivity     N/A
Concentration                   1 mg/ml
Form Liquid
Colour Clear
Preparation Ready to use
Storage Short term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze thaw cycles.

Storage Buffer 0.1M Phosphate Buffered Saline, pH 7.4
Preservative: None
Purification Notes This product was purified using Protein A- affinity chromatography.
Purity ≥ 95 % as determined by SDS-PAGE
General Notes If for any reason the product does not perform as specified, please contact our scientific support team for assistance by emailing techsupport@capebiologix.com.

For Research Use only, unless otherwise indicated.

Images

Figure 1. ELISA Dose Response curve: using PtX™ Mouse Anti-Delta-9-THC (Biotin) Antibody from 32– 0,00024 ng/uL to detect 50 ng THC-BSA antigen. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot: decreasing amounts of THC-BSA protein (monomer: 73 kDa, dimer at 140 kDa and tetramer at 260/280 kDa) detected with PtX™ Mouse Anti-Delta-9-THC (Biotin) at 1: 1 000 and Streptavidin-HRP conjugated at 1/5000.

PtX™ | Plant-Based Expression

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single-use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.

Visit our Technology page to learn more.

Important!

  • For Research Use Only.
  • Not for diagnostic or therapeutic use.
  • Not for resale without express authorization.
  • Trademark.

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