Mouse Anti-CTLA-4
Mouse Anti-CTLA-4 - Image 2

Mouse Anti-CTLA-4

Recombinant mouse monoclonal antibody against Cytotoxic T-lymphocyte-associated protein 4 (CTLA-4). Produced in Nicotiana benthamiana plants and suitable for ELISA applications.

100 µg      |     Catalogue Number: CBT_A0028

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Cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), also known as CD152, is a critical immune checkpoint receptor that plays a pivotal role in regulating T-cell activation and immune responses. By binding to the B7 ligands expressed on antigen-presenting cells, CTLA-4 acts as a negative regulator, downregulating T-cell activation and dampening immune responses. While this mechanism is crucial for preventing excessive immune reactions and autoimmune disorders, it can also hinder effective immune responses against cancer cells.  Our PtX™ Mouse Anti-CTLA-4 exhibits high specificity towards CTLA-4 and undergoes rigorous testing to ensure consistent performance and reliability. This specificity allows researchers to elucidate the specific roles of CTLA-4 in immunological processes. PtX™ Mouse Anti-CTLA-4 is suitable for use in Flow Cytometry and ELISA applications.

Additional information

Available Unit Size

0.1mg, 1mg, 10mg, 100mg, 200mg

Key Benefits

This product is a recombinant monoclonal antibody that offers several benefits including the following:

  • Batch-to-batch consistency.
  • No animals or animal products are used in the development of this protein.
  • BSA and Azide free.

For more information on plant-made, recombinant monoclonal antibodies click here.

Datasheet

Product Name PtX™ Mouse Anti-cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) Recombinant Antibody
Catalogue Number CBT_A0028
Expression Host Nicotiana benthamiana plants
Clonality Monoclonal, recombinant
Species and Isotype Mouse IgG2a
Tag None
Reporter Protein None
Description This product is a full-length Mouse IgG2 recombinant antibody that is specific to CTLA-4. It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens mediated infiltration.
Target Name Cytotoxic T-lymphocyte-associated protein 4 (CTLA-4)
Verified Applications Flow cytometry, ELISA
Dilution Range Flow Cytometry (1:300-1:500)

ELISA (1: 2 000 – 1: 10 000)
Concentration                   1.0 mg/ml
Form Liquid
Colour Clear
Preparation Ready to use
Storage Short term (up to one week): 2 – 8 °C
Long term: Aliquot and store at – 20 °C
Store immediately. Aliquot and avoid multiple freeze thaw cycles.
Storage Buffer 0.1  M Phosphate Buffered Saline, pH 7.4
Preservative: none
Purification Notes This product was purified using Protein A- affinity chromatography.
Purity ≥ 95% as determined by SDS-PAGE
General Notes If for any reason the product does not perform as specified, please contact our scientific support team for assistance by emailing techsupport@capebiologix.com.
For Research Use only, unless otherwise indicated.

Images

Figure 1. ELISA Dose Response curve using PtX™ Mouse Anti-CTLA-4 to detect coated CTLA-4 Antigen at 0.25 ng/µl and increasing concentrations of antibody. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Flow cytometry: Peripheral Blood Mononuclear cells (1.3 million cells) were fixed with 4% Paraformaldehyde and washed 2x in PBS buffer. Cells were stained with 1: 400 of PtXTM Mouse Anti-CTLA-4 Recombinant Antibody, followed by a commercial secondary Anti-Mouse conjugated to Alexa Fluor® 488 at 1: 400 dilution.  Approximately 30 000 events were acquired on a BD FACSDiva 8.0.2 Flow cytometer. A) Dot-plot and B) Histogram of the acquired 30 000 events.

PtX™ | Plant-Based Expression

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single-use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.

Visit our Technology page to learn more.

Important!

  • For Research Use Only.
  • Not for diagnostic or therapeutic use.
  • Not for resale without express authorization.
  • Trademark.

Feel free to contact our trusted distribution partners directly if you want to order this product.

 

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