Mouse Anti-CD47 (B6H12)

This product is a recombinant monoclonal antibody that offers several benefits including the following:

• Batch-to-batch consistency.
• This product is plant-made, no animals or animal products are used in the manufacture of this protein.
• BSA and Azide free.

For more information on plant-made, recombinant monoclonal antibodies click here.

Figure 1. ELISA Dose Response curve using PtX™ Mouse Anti-CD47 (B6H12) antibody from 0.3 – 0.00014 ng/µl to detect 25 ng Human CD47 protein. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot of decreasing amounts of Human CD47 protein (~35-44 kDa smear) detected with PtX^™ Mouse Anti-CD47 (B6H12) antibody at 1: 5 000 and an HRP conjugated anti-mouse secondary antibody at 1: 10 000.

Figure 3. Immunocytochemistry: HeLa cells were plated at 200 000 cells/ well in 6-well plates on coverslips and allowed to adhere. Following fixing and blocking, they were incubated with 1:200 dilution of Cape Biologix PtX^TM Mouse Anti-CD47 (B6H12) primary antibody (Cat# CBT_A0044), and 1:300 dilution of a commercial Anti-Mouse Alexa Fluor® 488 conjugated commercial secondary antibody. Images were taken on a Zeiss LSM780 with ELYRA PS1 platform confocal microscope (60X) at the Stellenbosch University CAF unit. Thank you to Prof Georgia Schafer (ICGEB) for kindly donating the HeLa cells and Mrs Lize Engelbrecht for her outstanding assistance.

Figure 4. Flow cytometry: Peripheral Blood Mononuclear cells (1.3 million cells) were fixed with 4% Paraformaldehyde and washed 2x in PBS buffer. Cells were stained with 1: 400 of PtX^TM Mouse Anti-CD47 Recombinant Antibody, followed by a commercial secondary Anti-Mouse conjugated to Alexa Fluor® 488 at 1: 400 dilution. Approximately 30 000 events were acquired on a BD FACSDiva 8.0.2 Flow cytometer. A) Dot-plot and B) Histogram of the acquired 30 000 events.

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single-use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.

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