Mouse Anti-CD28

Recombinant mouse monoclonal antibody against CD28. Produced in Nicotiana benthamiana plants and suitable for WB and ELISA applications.

100 µg      |     Catalogue Number: CBT_A0040

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Cluster of differentiation 28 (CD28), is a 44 kDa, type I transmembrane (glycoprotein) receptor, predominantly expressed on naïve T cells. Its role is to provide co-stimulatory signals required for T cell activation and survival. Through its counter-receptors; B7 ligands (CD80 and CD86), expressed by antigen presenting cells (APC), CD28 ligation has been shown to enhance the production of various cytokines, such as IL-2, IL-4, and IL-10, and has been implicated in the prevention of T cell anergy. The ligation further stabilizes Bcl-xL, an antiapoptotic molecule required for T cell survival. CD28 was one of the first molecules discovered to play a role in Treg-mediated control of autoimmunity. In essence, CD28-mediated co-stimulation greatly enhances the T cells effector response to antigen.

Additional information

Available Unit Size

0.1mg, 1mg, 10mg, 100mg, 200mg

Key Benefits

This product is a recombinant monoclonal antibody that offers several benefits including the following:

  • Batch-to-batch consistency.
  • This product is plant-made, no animals or animal products are used in the manufacture of this protein.
  • BSA and Azide free.

For more information on plant-made, recombinant monoclonal antibodies click here.

Datasheet

Product Name PtX™ Mouse Anti-CD28 Recombinant Antibody
Catalogue Number CBT_A0040
Expression Host Nicotiana benthamiana plants
Clonality Monoclonal, recombinant
Species and Isotype Mouse IgG2a
Tag None
Reporter Protein None
Description This product is a full-length Mouse IgG2a recombinant antibody specific to the CD28 antigen. It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens mediated infiltration.
Target Name CD28
Verified Applications Western blot, ELISA
Dilution Range Western blot (1: 1 000 – 1: 6 000), ELISA (1: 500 – 1: 8 000)
Concentration 1.0 mg/ml
Form Liquid
Colour Clear
Preparation Ready to use
Storage Short-term (up to one week): 2 – 8 °C
Long term: Aliquot and store at – 20 °C
Store immediately. Aliquot and avoid multiple freeze-thaw cycles.
Storage Buffer 0.1  M Phosphate Buffered Saline, pH 7.4
Preservative: none
Purification Notes This product was purified using Protein A- affinity chromatography.
Purity ≥ 95 % as determined by SDS-PAGE.
General Notes If for any reason the product does not perform as specified, please contact our scientific support team for assistance by emailing sales@capebiologix.com.

 

Images

Figure 1. ELISA Dose Response curve using PtX Mouse Anti-CD28 from 4 – 0.0002 ng/µl to detect 25 ng CD28 antigen. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot of decreasing amounts of CD28 Antigen (35-50 kDa) detected with PtX Mouse Anti-CD28 at 1: 2 000 and an HRP conjugated anti-mouse secondary antibody at 1: 10 000.

Figure 3. ELISA Dose Response curve detecting 25 ng CD28 antigen using PtX Mouse Anti-CD28 Antibody from 4 – 0.0002 ng/µl stored at -20°C and shipping conditions for two weeks. For shipping conditions, the product is placed under conditions that simulate real time shipping conditions. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

PtX™ | Plant-Based Expression

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single-use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.

Visit our Technology page to learn more.

Important!

  • For Research Use Only.
  • Not for diagnostic or therapeutic use.
  • Not for resale without express authorization.
  • Trademark.

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