Mouse Anti-CD20 (1F5)

Recombinant mouse monoclonal antibody against CD20. Produced in Nicotiana benthamiana plants and suitable for ELISA application.

100 µg      |     Catalogue Number: CBT_A0029

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CD20 is a cell surface protein expressed predominantly on B cells, playing a crucial role in their development, activation, and differentiation. It is involved in regulating B cell signalling and is implicated in B cell-mediated immune responses. CD20 is an appealing target for research due to its selective expression on B cells, making it a potential therapeutic target for B cell malignancies and autoimmune disorders. Our PtX™ Mouse Anti-CD20 (1F5) antibody enables researchers to investigate the distribution, activation, and maturation of B cells in different physiological and pathological contexts. This antibody is rigorously validated to ensure high specificity, sensitivity, and reproducibility, and is suitable for ELISA blot applications.

Additional information

Available Unit Size

0.1mg, 1mg, 10mg, 100mg, 200mg

Key Benefits

This product is a recombinant monoclonal antibody that offers several benefits including the following:

  • Batch-to-batch consistency.
  • No animals or animal products are used in the development of this protein.
  • BSA and Azide free.

For more information on plant-made, recombinant monoclonal antibodies click here.

Datasheet

Product Name PtX™ Mouse Anti-CD20 (1F5) Recombinant Antibody.
Catalogue Number CBT_A0029
Expression Host Nicotiana benthamiana plants
Clonality Monoclonal, recombinant
Species and Isotype Mouse IgG2a
Tag None
Reporter Protein None
Description This product is a full-length Mouse IgG2a recombinant antibody specific to the human B-lymphocyte antigen CD20. It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens mediated infiltration.
Target Name CD20
Verified Applications ELISA
Dilution Range ELISA (1: 1 000 – 1: 64 000)
Tested Species Reactivity  Human
Concentration                   1.0 mg/ml
Form Liquid
Colour Clear to light yellow
Preparation Ready to use
Storage Short term (up to one week): 2 – 8 °C
Long term: Aliquot and store at – 20 °C
Store immediately. Aliquot and avoid multiple freeze thaw cycles.
Storage Buffer 0.1  M Phosphate Buffered Saline, pH 7.4
Preservative: None
Purification Notes This product was purified using Protein A affinity chromatography.
Purity ≥ 95 % as determined by SDS-PAGE
General Notes If for any reason the product does not perform as specified, please contact our scientific support team for assistance by emailing sales@capebiologix.com.

Images

Figure 1. ELISA Dose Response curve using PtX™ Mouse Anti-CD20 (1F5) to detect coated Human B-lymphocyte antigen CD20 Antigen at 1 ng/µl and increasing concentrations of antibody. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. ELISA Dose Response curve using PtX™ Mouse Anti-CD20 (1F5) to detect coated Human B-lymphocyte stored at -20°C and shipping conditions for two weeks. At shipping the product is placed under conditions that simulate real time shipping conditions. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

 

PtX™ | Plant-Based Expression

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single-use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.

Visit our Technology page to learn more.

Important!

  • For Research Use Only.
  • Not for diagnostic or therapeutic use.
  • Not for resale without express authorization.
  • Trademark.

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