Mouse Anti-c-Myc (9E10)

Recombinant mouse monoclonal antibody against proto-oncogene; c-Myc and other c-Myc tagged proteins. This product is produced in Nicotiana benthamiana plants and is suitable for Western Blot and ELISA applications.

100 µg      |     Catalogue Number: CBT_A0047

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c-Myc, short for cellular Myelocytomatosis oncogene, is a proto-oncogene that plays a critical role in regulating cell growth, proliferation, and differentiation. It belongs to the Myc family of transcription factors and is found in all human cells.  c-Myc also plays a crucial role in cellular metabolism, stimulating the uptake and utilization of nutrients to support the high energy demands of rapidly dividing cells. Additionally, c-Myc influences cell differentiation and apoptosis, making it a key regulator of normal development and tissue homeostasis. In cancer cells, the uncontrolled expression of c-Myc promotes abnormal cell growth, survival, and invasion. Altered c-Myc expression is therefore strongly associated with tumor formation and is required for vasculogenesis and angiogenesis within tumor tissue. PtX™ Mouse Anti-c-Myc (9E10) specifically detects c-Myc protein, as well as c-Myc-tagged recombinant proteins, and has been validated for use in WB and ELISA.

Additional information

Available Unit Size

0.1mg, 1mg, 10mg, 100mg, 200mg

Key Benefits

This product is a recombinant monoclonal antibody that offers several benefits including the following:

  • Batch-to-batch consistency.
  • This product is plant-made, no animals or animal products are used in the manufacture of this protein.
  • BSA and Azide free.

For more information on plant-made, recombinant monoclonal antibodies click here.

Datasheet

Product Name PtX™ Mouse Anti-c-Myc (9E10) Recombinant Antibody
Catalogue Number CBT_A0047
Expression Host Nicotiana benthamiana plants
Clonality Monoclonal, Recombinant
Species and Isotype Mouse IgG2a
Tag None
Reporter Protein None
Description This product is a full-length Mouse IgG2a recombinant antibody specific to His & c-Myc Tagged-rSRGN antigen. It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.
Target Name c-Myc
Verified Applications Western blot, ELISA, ICC
Recommended Dilutions Western Blot (1: 1 000 – 1: 5 000)

ELISA (1: 500 – 1: 5 000)

ICC (1:100-1:300)
Concentration 1.0 mg/ml
Form Liquid
Colour Clear
Preparation Ready to use
Storage Short-term (up to one week): 2 – 8 °C
Long term: Aliquot and store at – 20 °C
Store immediately. Aliquot and avoid multiple freeze-thaw cycles.
Storage Buffer 0.1 M Phosphate Buffered Saline, pH 7.4
Preservative: none
Purification Notes This product was purified using Protein A- affinity chromatography.
Purity ≥ 95 % as determined by SDS-PAGE.
General Notes If for any reason the product does not perform as specified, please contact our scientific support team for assistance by emailing techsupport@capebiologix.com.
For Research Use only, unless otherwise indicated.

 

Images

Figure 1. ELISA Dose Response curve using PtX™ Mouse Anti-c-Myc (9E10) from 4 – 0.0002441 ng/uL to detect 0.1 ng c-Myc antigen. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot of decreasing amounts of c-Myc antigen (25 kDa) detected with PtX™ Mouse Anti-c-Myc (9E10) at 1: 1 000 and an HRP conjugated anti-mouse secondary antibody.

Figure 3. Immunocytochemistry: HeLa cells were plated at 200 000 cells/ well in 6-well plates on coverslips and allowed to adhere. Following fixation and blocking, they were incubated with 1:200 dilution of Cape Biologix PtX™ Mouse Anti-c-Myc primary antibody (Cat# CBT_A0047), and 1:300 dilution of a commercial Anti-Mouse Alexa Fluor® 488 conjugated commercial secondary antibody. Cells were stained with nuclear stain Hoechst 33342 for 5 minutes. Images were taken on a Zeiss LSM780 with ELYRA PS1 platform confocal microscope (20X) at the Stellenbosch University CAF unit. Thank you to Prof Georgia Schafer (ICGEB) for kindly donating the HeLa cells and Mrs Lize Engelbrecht for her outstanding assistance.

PtX™ | Plant-Based Expression

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single-use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.

Visit our Technology page to learn more.

Important!

  • For Research Use Only.
  • Not for diagnostic or therapeutic use.
  • Not for resale without express authorization.
  • Trademark.

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