PtX™ Mouse Anti-c-Myc (9E10)

Recombinant mouse monoclonal antibody against proto-oncogene; c-Myc and other c-Myc tagged proteins. This product is produced in Nicotiana benthamiana plants and is suitable for Western Blot and ELISA applications.

100 µg      |     Catalogue Number: CBT_A0047

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c-Myc, short for cellular Myelocytomatosis oncogene, is a proto-oncogene that plays a critical role in regulating cell growth, proliferation, and differentiation. It belongs to the Myc family of transcription factors and is found in all human cells.  c-Myc also plays a crucial role in cellular metabolism, stimulating the uptake and utilization of nutrients to support the high energy demands of rapidly dividing cells. Additionally, c-Myc influences cell differentiation and apoptosis, making it a key regulator of normal development and tissue homeostasis. In cancer cells, the uncontrolled expression of c-Myc promotes abnormal cell growth, survival, and invasion. Altered c-Myc expression is therefore strongly associated with tumor formation and is required for vasculogenesis and angiogenesis within tumor tissue. PtX™ Mouse Anti-c-Myc (9E10) specifically detects c-Myc protein, as well as c-Myc-tagged recombinant proteins, and has been validated for use in WB and ELISA.

Additional information

Available Unit Size

0.1mg, 1mg, 10mg, 100mg, 200mg

Key Benefits

This product is a recombinant monoclonal antibody that offers several benefits including the following:

  • Batch-to-batch consistency.
  • This product is plant-made, no animals or animal products are used in the manufacture of this protein.
  • BSA and Azide free.

For more information on plant-made, recombinant monoclonal antibodies click here.

Datasheet

Product Name PtX™ Mouse Anti-c-Myc (9E10) Recombinant Antibody
Catalogue Number CBT_A0047
Expression Host Nicotiana benthamiana plants
Clonality Monoclonal, Recombinant
Species and Isotype Mouse IgG2a
Tag None
Reporter Protein None
Description This product is a full-length Mouse IgG2a recombinant antibody specific to His & c-Myc Tagged-rSRGN antigen. It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.
Target Name c-Myc
Verified Applications Western blot, ELISA
Recommended Dilutions Western Blot (1: 1 000 – 1: 5 000), ELISA (1: 500 – 1: 5 000)
Concentration 1.0 mg/ml
Form Liquid
Colour Clear
Preparation Ready to use
Storage Short-term (up to one week): 2 – 8 °C
Long term: Aliquot and store at – 20 °C
Store immediately. Aliquot and avoid multiple freeze-thaw cycles.
Storage Buffer 0.1  M Phosphate Buffered Saline, pH 7.4
Preservative: none
Purification Notes This product was purified using Protein A- affinity chromatography.
Purity ≥ 95 % as determined by SDS-PAGE.
General Notes If for any reason the product does not perform as specified, please contact our scientific support team for assistance by emailing sales@capebiologix.com.

 

Images

Figure 1. ELISA Dose Response curve using PtX™ Mouse Anti-c-Myc (9E10) from 4 – 0.0002441 ng/uL to detect 0.1 ng c-Myc antigen. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot of decreasing amounts of c-Myc antigen (25 kDa) detected with PtX™ Mouse Anti-c-Myc (9E10) at 1: 1 000 and an HRP conjugated anti-mouse secondary antibody.

Figure 3. ELISA Dose Response curve detecting 0.1 ng c-Myc antigen using PtX™ Mouse Anti-c-Myc (9E10) from 4 – 0.0002441 ng/uL stored at -20°C and shipping conditions for two weeks. For shipping the product is placed under conditions that simulate real time shipping conditions. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

 

PtX™ | Plant-Based Expression

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single-use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.

Visit our Technology page to learn more.

Important!

  • For Research Use Only.
  • Not for diagnostic or therapeutic use.
  • Not for resale without express authorization.
  • Trademark.

Feel free to contact our trusted distribution partners directly if you want to order this product.

 

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