Human Anti-IL-2 (1F90)

Recombinant human monoclonal antibody against IL-2. This product is produced in Nicotiana benthamiana plants for use in ELISA and Western blot applications.

100 µg      |     Catalogue Number: CBT_A0045

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Interleukin-2 (IL-2) is a cytokine that plays a critical role in regulating the immune system. It is primarily produced by activated T cells and acts as a key mediator of immune responses. IL-2 functions by binding to specific receptors on various immune cells, including T cells, B cells, and natural killer (NK) cells, which triggers a cascade of signalling events that promote the activation, proliferation, and differentiation of these immune cells. Additionally, IL-2 promotes the development and maintenance of regulatory T cells (Tregs), which play a crucial role in immune tolerance and preventing autoimmune responses. IL-2 also supports the differentiation of NK cells, boosting their cytotoxic activity against target cells. In cancer immunotherapy, IL-2 has been used to stimulate the immune system and enhance the antitumor activity of T cells and NK cells. Furthermore, IL-2 has been studied to understand its impact on the development of immunological memory and as a potential adjuvant in vaccine design. The stringent quality control processes behind our PtX™ Human anti-IL-2 antibody ensures optimal performance and reproducibility. Its application potential and reliable performance make it an invaluable tool for scientists, clinicians, and researchers dedicated to advancing immunotherapy, immune regulation, and the understanding of immune-related diseases.

Additional information

Available Unit Size

0.1mg, 1mg, 10mg, 100mg, 200mg

Key Benefits

This product is a recombinant monoclonal antibody that offers several benefits including the following:

  • Batch-to-batch consistency.
  • This product is plant-made, no animals or animal products are used in the manufacture of this protein.
  • BSA and Azide free.

For more information on plant-made, recombinant monoclonal antibodies click here.

Datasheet

Product Name PtX™ Human Anti-Interleukin-2 (1F90) Recombinant Antibody
Catalogue Number CBT_A0045
Expression Host Nicotiana benthamiana plants
Clonality Monoclonal, Recombinant
Species and Isotype Human IgG1
Tag None
Reporter Protein None
Description This product is a full-length Human IgG1 recombinant antibody specific to Interleukin-2. It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens mediated infiltration.
Target Name Human Interleukin-2
Verified Applications Western blot, ELISA
Dilution Range Western Blot (1: 500 – 1: 1 000), ELISA (1: 375 – 1: 48 000)
Tested Species Reactivity     Human
Concentration 1.0 mg/ml
Form Liquid
Colour Clear
Preparation Ready to use
Storage Short-term (up to one week): 2 – 8 °C
Long term: Aliquot and store at – 20 °C
Store immediately. Aliquot and avoid multiple freeze-thaw cycles.
Storage Buffer 0.1  M Phosphate Buffered Saline, pH 7.4
Preservative: none
Purification Notes This product was purified using Protein A- affinity chromatography.
Purity ≥ 95 % as determined by SDS-PAGE.
General Notes If for any reason the product does not perform as specified, please contact our scientific support team for assistance by emailing sales@capebiologix.com.

 

Images

Figure 1. ELISA Dose Response curve using PtX™ Human Anti-IL-2 (1F90) antibody from 3 – 0.00137 ng/µl to detect 25 ng Human Interleukin-2 protein. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot of decreasing amounts of Human Interleukin-2 protein (18.4 and 16.6 kDa) detected with PtX™ Human Anti-IL-2 (1F90) antibody at a 1: 500 dilution and an HRP conjugated anti-human secondary antibody at 1: 10 000.

Figure 3. ELISA Dose Response curve to detect 25 ng of Human Interleukin-2 protein using PtX™ Human Anti-IL-2 (1F90) antibody from 3 – 0.00137 ng/µl stored at -20°C and shipping conditions for two weeks. At shipping the product is placed under conditions that simulate real time shipping conditions. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

 

PtX™ | Plant-Based Expression

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single-use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.

Visit our Technology page to learn more.

Important!

  • For Research Use Only.
  • Not for diagnostic or therapeutic use.
  • Not for resale without express authorization.
  • Trademark.

Feel free to contact our trusted distribution partners directly if you want to order this product.

 

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