Human Anti-CD28 (HRP) Antibody

This product is a full-length Human IgG1 recombinant antibody specific to CD28, fused at the genetic level to HRP reporter enzyme. It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.

100 µg      |     Catalogue Number: CBT_A0063

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Cluster of differentiation 28 (CD28), is a 44 kDa, type I transmembrane (glycoprotein) receptor, predominantly expressed on naïve T cells. Its role is to provide co-stimulatory signals required for T cell activation and survival. Through its counter-receptors; B7 ligands (CD80 and CD86), expressed by antigen presenting cells (APC), CD28 ligation has been shown to enhance the production of various cytokines, such as IL-2, IL-4, and IL-10, and has been implicated in the prevention of T cell anergy. The ligation further stabilizes Bcl-xL, an antiapoptotic molecule required for T cell survival. CD28 was one of the first molecules discovered to play a role in Treg-mediated control of autoimmunity. In essence, CD28-mediated co-stimulation greatly enhances the T cells effector response to antigen. 

Additional information

Available Unit Size

0.1mg, 1mg, 10mg, 100mg, 200mg

Key Benefits

This product is a recombinant monoclonal antibody that offers several benefits including the following:

  • Enhanced signal intensity and sensitivity
  • Reduced background noise
  • Compatibility with multiple species and antibody isotypes
  • Minimized steric hindrance.
  • Batch-to-batch consistency.
  • No animals or animal products are used in the development of this protein.
  • BSA and Azide free.

For more information on plant-made, recombinant monoclonal antibodies click here. 

Datasheet

Product Name PtX™ Human Anti-CD28 (HRP) Recombinant Antibody
Catalogue Number CBT_A0063
Expression Host Nicotiana benthamiana plants
Clonality Monoclonal, recombinant
Species and Isotype Human IgG1
Tag None
Reporter Protein Horseradish Peroxidase (HRP)
Description This product is a full-length Human IgG1 recombinant antibody specific to CD28, fused to HRP at the genetic level. It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.
Target Name CD28
Verified Applications ELISA, Western blot
Dilution Range ELISA (1: 1 000 – 1: 64 000)

Western blot (1: 1 000 – 1: 2 000)
Tested Species Reactivity     Human
Concentration                   1 mg/ml
Form Liquid
Colour Clear
Preparation Ready to use
Storage Short term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze thaw cycles.
Storage Buffer 0.1M Phosphate Buffered Saline, pH 7.4
Purification Notes This product was purified using Protein A- affinity chromatography.
Purity ≥ 95 % as determined by SDS-PAGE
General Notes If for any reason the product does not perform as specified, please contact our scientific support team for assistance by emailing techsupport@capebiologix.com.

For Research Use only, unless otherwise indicated.

Images

Figure 1. ELISA Dose Response curve using PtX™ Human Anti-CD28 (HRP) Antibody from 0.0002 – 4.0 ng/uL to detect 50 ng Recombinant human CD28 protein. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot of decreasing amounts of Recombinant human CD28 protein (15 kDa and can migrate as a ‘smear’ ~37-50 kDa) detected with PtX™ Human Anti-CD28 (HRP) Antibody at 1: 1 000.

PtX™ | Plant-Based Expression

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single-use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.

Visit our Technology page to learn more.

Important!

  • For Research Use Only.
  • Not for diagnostic or therapeutic use.
  • Not for resale without express authorization.
  • Trademark.

Feel free to contact our trusted distribution partners directly if you want to order this product.

 

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