Rabbit Anti-GST (Biotin)

This product is a full-length biotinylated rabbit IgG1 recombinant antibody specific to Glutathione-S-Transferase (GST). It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.

100 µg      |     Catalogue Number: CBT_A0001[B]

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Glutathione S-transferase (GST) is a detoxification enzyme which catalyses the conjugation of the reduced form of glutathione to hydrophobic and electrophilic compounds of xenobiotic substrates, thereby protecting the host against toxic foreign chemicals and oxidative stress. The GST superfamily can be categorized into cytosolic, mitochondrial and microsomal GST subfamilies. GST-tags are often fused to target recombinant proteins to facilitate the purification and study of the target proteins by antibodies directed against the GST tags.

The biotin labelling of this antibody ensures strong and stable interaction with avidin or streptavidin, making it suitable for a wide range of downstream applications, including Western blotting and ELISA.

Additional information

Available Unit Size

0.1mg, 1mg, 10mg, 100mg, 200mg

Key Benefits

This product is a plant-made, recombinant monoclonal antibody, which offers several advantages including:

  • Can detect recombinant GST in native and denatured forms.
  • Protein is fused at the genetic level for increased reproducibility of results.
  • No animals or animal products are used in the development of this protein.

Datasheet

Product Name PtX™ Rabbit Anti-GST Biotinylated Recombinant Antibody
Catalogue Number CBT_A0001[B]
Expression Host Nicotiana benthamiana plants
Clonality Monoclonal, recombinant
Species and Isotype Rabbit IgG1
Tag Biotin
Reporter Protein None
Description This product is a full-length biotinylated Rabbit IgG1 recombinant antibody specific to Glutathione-S-Transferase (GST). It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.
Target Name Glutathione-S-Transferase (GST)
Verified Applications Western blot, ELISA
Dilution Range Western blot (1: 1 000 – 1: 10 000)

ELISA (1: 1 000 – 1: 1 000 000)
Tested Species Reactivity     Human
Concentration                   1 mg/ml
Form Liquid
Colour Clear
Preparation Ready to use
Storage Short term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze thaw cycles.
Storage Buffer 0.1M Phosphate Buffered Saline, pH 7.4
Preservative: None
Purification Notes This product was purified using Protein A- affinity chromatography.
Purity 95 % as determined by SDS-PAGE
General Notes If for any reason the product does not perform as specified, please contact our scientific support team for assistance by emailing techsupport@capebiologix.com.

For Research Use only, unless otherwise indicated.

Images

Figure 1. ELISA Dose Response curve: PtX™ Rabbit Anti-GST (Biotin) Antibody from 0.0002– 4.0 ng/uL to detect 25 ng GST antigen. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot: decreasing amounts of GST protein (28 kDa) detected with PtX™ Rabbit Anti-GST (Biotin) Antibody at 1: 10 000 and HRP conjugated Streptavidin secondary antibody at 1: 5 000.

PtX™ | Plant-Based Expression

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single-use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.

Visit our Technology page to learn more.

Important!

  • For Research Use Only.
  • Not for diagnostic or therapeutic use.
  • Not for resale without express authorization.
  • Trademark.

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