Rabbit Anti-c-Myc (eGFP)

This product is a full-length Rabbit IgG1 recombinant antibody specific to c-Myc, fused at the genetic level to eGFP. It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.

100 µg      |     Catalogue Number: CBT_A0059

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Recombinant rabbit monoclonal antibody against proto-oncogene; c-Myc and other c-Myc tagged proteins. This product is produced in Nicotiana benthamiana plants and is suitable for Western Blot and ELISA applications. It is fused to eGFP, allowing applications such as fluorescent Western blots and Immunocytochemistry.

C-Myc is a 62 kDa transcription factor known for its role in cell division, differentiation, and apoptosis. Altered c-Myc expression is associated with tumor formation and is required for vasculogenesis and angiogenesis within tumor tissue.

PtX™ Rabbit Anti-c-Myc (9E10) (eGFP), CBT_A0007[B], detects c-Myc protein, as well as c-Myc-tagged recombinant proteins, and has been validated for use in WB and ELISA.

 

Additional information

Available Unit Size

0.1mg, 1mg, 10mg, 100mg, 200mg

Key Benefits

This product is a recombinant monoclonal antibody that offers several benefits including the following:

  • Batch-to-batch consistency.
  • Capable of detecting c-Myc and recombinant proteins fused to c-Myc tags, upwards of 2.5ng.
  • No animals or animal products are used in the development of this protein.
  • BSA and Azide free.

Datasheet

Product Name PtX™ Rabbit Anti-c-Myc (9E10) (eGFP) Recombinant Antibody
Catalogue Number CBT_A0059
Expression Host Nicotiana benthamiana plants
Clonality Monoclonal, recombinant
Species and Isotype Rabbit IgG1
Tag Enhanced green fluorescent protein (eGFP)
Reporter Protein None
Description This product is a full-length Rabbit IgG1 recombinant antibody specific to c-Myc and fused at the genetic level to eGFP. It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.
Target Name c-Myc
Verified Applications Western blot, ELISA, fluorescent western blot
Dilution Range Western blot (1: 1 000 – 1: 5 000)

ELISA (1: 1 000 – 1: 512 000)

Fluorescent western blot (1: 1 000 – 1: 2 000)
Tested Species Reactivity     Human
Concentration                   1 mg/ml
Form Liquid
Colour Clear with a lime green tinge
Preparation Ready to use
Storage Short term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze thaw cycles.
Storage Buffer 0.1 M Tris, 0.09 M Glycine, pH 8
Purification Notes This product was purified using Protein A- affinity chromatography.
Purity >95 % as determined by SDS-PAGE
General Notes If for any reason the product does not perform as specified, please contact our scientific support team for assistance by emailing techsupport@capebiologix.com.

For Research Use only, unless otherwise indicated.

Images

Figure 1. ELISA Dose Response curve: PtX™ Rabbit Anti-c-Myc (eGFP) Antibody from 0.000032– 2.0 ng/uL to detect 10 ng Human Serglycin/SRGN Protein (His & Myc Tag) antigen. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

 

Figure 2. Western blot: decreasing amounts of Human Serglycin/SRGN Protein (c-Myc Tagged) (20 kDa) detected with PtX™ Rabbit Anti-c-Myc (eGFP) at 1: 1 000 and an HRP conjugated anti-Rabbit secondary antibody at 1: 10 000.

 

PtX™ | Plant-Based Expression

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single-use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.

Visit our Technology page to learn more.

Important!

  • For Research Use Only.
  • Not for diagnostic or therapeutic use.
  • Not for resale without express authorization.
  • Trademark.

Feel free to contact our trusted distribution partners directly if you want to order this product.

 

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