Human Anti-TNF Alpha (Biotin)

This product is a full-length biotinylated Human IgG1 recombinant antibody specific to TNF Alpha. It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.

100 µg      |     Catalogue Number: CBT_A0013[B]

Download: SDS / Datasheet

View Product


TNF-α (tumor necrosis factor alpha) is a pro-inflammatory cytokine that is produced and released by various cell types, including T-cells, macrophages, and monocytes. TNF-α acts by binding to specific receptors on the surface of target cells, which leads to the activation of signaling pathways that result in inflammation, cell death, and tissue destruction. It also plays a role in the development of cancer and the progression of autoimmune diseases such as rheumatoid arthritis and Crohn's disease. Targeting TNF-α has become a major therapeutic strategy for treating cancer and inflammatory diseases.

The high specificity and binding affinity of our PtX™ Mouse Anti-Tumor Necrosis Factor Alpha Recombinant Antibody makes it an ideal tool for researchers studying TNF alpha-mediated signaling pathways and its potential as a therapeutic target. The biotin labeling of this antibody ensures strong and stable interaction with avidin or streptavidin, making it suitable for a wide range of downstream applications, including Western blotting and ELISA. This product is suitable for research use only and not for use in humans.

Additional information

Available Unit Size

0.1mg, 1mg, 10mg, 100mg, 200mg

Key Benefits

This product is a recombinant monoclonal antibody that offers several benefits including the following: 

  • Enhanced signal intensity and sensitivity
  • Reduced background noise
  • Compatibility with multiple species and antibody isotypes
  • Minimized steric hindrance.
  • Batch-to-batch consistency
  • This product is plant-made, no animals or animal products are used in the manufacture of this antibody.
  • BSA and Azide free

For more information on plant-made, recombinant monoclonal antibodies click here. 


Product Name PtX™ Human Anti-TNF Alpha (Biotin) Recombinant Antibody
Catalogue Number CBT_A0013[B]
Expression Host Nicotiana benthamiana plants
Clonality Monoclonal, recombinant
Species and Isotype Human IgG1
Tag Biotin
Reporter Protein None
Description This product is a full-length Human IgG1 recombinant antibody specific to TNF Alpha. It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.
Target Name Human Tumor Necrosis Factor Alpha (TNFα)
Verified Applications Western blot, ELISA
Dilution Range Western blot (1: 1 000 – 1: 10 000)

ELISA (1: 1 000 – 1: 250 000)

Tested Species Reactivity     Human
Concentration                   1 mg/ml
Form Liquid
Colour Clear
Preparation Ready to use
Storage Short term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze thaw cycles.

Storage Buffer 0.1M Phosphate Buffered Saline, pH 7.4
Purification Notes This product was purified using Protein A- affinity chromatography.
Purity ≥ 95 % as determined by SDS-PAGE
General Notes If for any reason the product does not perform as specified, please contact our scientific support team for assistance by emailing

For Research Use only, unless otherwise indicated.


Figure 1.ELISA Dose Response curve: PtX™ Human Anti-TNF Alpha (Biotin) Antibody from 0.0002 – 4.0 ng/uL to detect 25 ng TNF Alpha antigen. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot: decreasing amounts of TNF Alpha (17 kDa) detected with PtX™ Human Anti-TNF Alpha (Biotin) at 1: 2 000 and HRP conjugated Streptavidin at 1: 5 000.

PtX™ | Plant-Based Expression

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single-use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.

Visit our Technology page to learn more.


  • For Research Use Only.
  • Not for diagnostic or therapeutic use.
  • Not for resale without express authorization.
  • Trademark.

Feel free to contact our trusted distribution partners directly if you want to order this product.


Contact our Distribution Partners

  Sign up for our newsletter

Click Here