Mouse Anti-IL-8 (Biotin)

This product is a full-length Mouse IgG2a recombinant antibody specific to Interleukin 8 (IL-8), conjugated to Biotin. It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.

100 µg      |     Catalogue Number: CBT_A0033[B]

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Interleukin-8 (IL-8, CXCL8) is a pro-inflammatory cytokine that is a member of the chemokine family. Cytokines are small signalling molecules that are secreted by immune cells and other cell types in response to infection, injury, or other stimuli. IL-8 is produced by a variety of cell types, including immune cells, endothelial cells, and epithelial cells, and plays an important role in the immune response to infection and inflammation. IL-8 binds to specific receptors on the surface of neutrophils, called CXCR1 and CXCR2, which activate signaling pathways within the cells that lead to their migration towards the site of infection or inflammation. In addition, IL-8 also activate other immune cells such as T cells and natural killer cells and promote angiogenesis in tissues undergoing repair or remodelling. Excessive or chronic production of IL-8 can contribute to the pathogenesis of a variety of inflammatory and autoimmune diseases, such as rheumatoid arthritis, chronic obstructive pulmonary disease (COPD), and cancer.

Our recombinant mouse anti-IL-8 antibody is a high-affinity, monoclonal antibody that specifically recognizes and binds to IL-8. The biotin labelling of this antibody ensures strong and stable interaction with avidin or streptavidin, making it suitable for a wide range of downstream applications, including Western blotting and ELISA. PtX™ Mouse Anti-IL-8 (Biotin) is an ideal tool for investigating the role of IL-8 in disease pathogenesis and for developing novel therapeutic strategies targeting this protein.

Additional information

Available Unit Size

0.1mg, 1mg, 10mg, 100mg, 200mg

Key Benefits

This product is a recombinant monoclonal antibody that offers several benefits including the following:

  • Enhanced signal intensity and sensitivity
  • Reduced background noise
  • Compatibility with multiple species and antibody isotypes
  • Minimized steric hindrance
  • Batch-to-batch consistency
  • This product is plant-made, no animals or animal products are used in the manufacture of this antibody
  • BSA and Azide free

For more information on plant-made, recombinant monoclonal antibodies click here.

Datasheet

Product Name PtX™ Mouse Anti-Interleukin-8 (Biotin) Recombinant Antibody
Catalogue Number CBT_A0033[B]
Expression Host Nicotiana benthamiana plants
Clonality Monoclonal, recombinant
Species and Isotype Mouse IgG2a
Tag Biotin
Reporter Protein None
Description This product is a full-length Mouse IgG2a recombinant antibody specific to Interleukin 8 (IL-8). It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.
Target Name Interleukin 8 (IL-8)
Verified Applications Western blot, ELISA
Dilution Range Western blot (1: 1 000 – 1: 10 000)

ELISA (1: 1 000 – 1: 100 000)
Tested Species Reactivity     Human
Concentration                   1 mg/ml
Form Liquid
Colour Clear
Preparation Ready to use
Storage Short term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze thaw cycles.
Storage Buffer 0.1 M Phosphate Buffered Saline, pH 7.4
Purification Notes This product was purified using Protein A- affinity chromatography.
Purity >95 % as determined by SDS-PAGE
General Notes If for any reason the product does not perform as specified, please contact our scientific support team for assistance by emailing techsupport@capebiologix.com.

For Research Use only, unless otherwise indicated.

Images

Figure 1. ELISA Dose Response curve using PtX™ Mouse Anti-IL-8 (Biotin) Antibody from 4.0 – 0,00024 ng/uL to detect 10 ng IL-8 (Human IL-8/CXCL8 Protein (His tag) antigen. Experiments were performed in duplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot of decreasing amounts of Human IL-8/CXCL8 Protein (His tag) (12 kDa) detected with PtX™ Mouse Anti-IL-8 (Biotin) at 1: 5 000 and Streptavidin-HRP at 1: 5 000.

PtX™ | Plant-Based Expression

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single-use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.

Visit our Technology page to learn more.

Important!

  • For Research Use Only.
  • Not for diagnostic or therapeutic use.
  • Not for resale without express authorization.
  • Trademark.

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