Mouse Anti-HA Tag

This product is a full-length Mouse IgG2a recombinant antibody specific to HA Tag. It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.

100 µg      |     Catalogue Number: CBT_A0050

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HA tag is derived from amino acid residues 98 – 106 of the human influenza hemagglutinin (HA) molecule. Recombinant proteins are often engineered to express the HA tag as a general epitope tag in expression vectors. HA tag facilitates detection, isolation, and purification of target proteins without compromising its bioactivity or distribution. HA-tagging of a recombinant protein and subsequent examination using anti-HA antibody, in either intact or permeabilized cells, allows researchers to study cellular expression levels, topology and functional activity of the HA-tagged protein. Antibody mediated detection of the HA tagged proteins further negates the need for developing specific antibodies for newly identified proteins, which are often unstable, difficult to purify, cross-reactive or poorly immunogenic proteins. Our PtX™ Mouse Anti-HA antibody exhibits high specificity towards HA-tagged proteins and undergoes rigorous testing to ensure consistent performance and reliability.

Additional information

Available Unit Size

0.1mg, 1mg, 10mg, 100mg, 200mg

Key Benefits

This product is a recombinant monoclonal antibody that offers several benefits including the following:

  • Batch-to-batch consistency.
  • No animals or animal products are used in the development of this protein.
  • BSA and Azide free.

For more information on plant-made, recombinant monoclonal antibodies click here.

Datasheet

Product Name PtX™ Mouse Anti-Hemagglutinin Tag Recombinant Antibody
Catalogue Number CBT_A0050
Expression Host Nicotiana benthamiana plants
Clonality Monoclonal, recombinant
Species and Isotype Mouse IgG2a
Tag None
Reporter Protein None
Description This product is a full-length Mouse IgG2a recombinant antibody specific to Hemagglutinin Tag (HA Tag). It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.
Target Name Hemagglutinin Tag (HA Tag)
Verified Applications Western blot, ELISA
Dilution Range Western blot (1: 1 000 – 1: 2 000)

ELISA (1: 500 – 1: 1 000)
Tested Species Reactivity     Human
Concentration                   1 mg/ml
Form Liquid
Colour Clear
Preparation Ready to use
Storage Short term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze thaw cycles.
Storage Buffer 0.1M Phosphate Buffered Saline, pH 7.4
Purification Notes This product was purified using Protein A- affinity chromatography.
Purity 95 % as determined by SDS-PAGE
General Notes If for any reason the product does not perform as specified, please contact our scientific support team for assistance by emailing techsupport@capebiologix.com.

For Research Use only, unless otherwise indicated.

Images

Figure 1. ELISA Dose Response curve using PtX™ Mouse Anti-HA tag Antibody from 0.5–0.0000305 ng/uL to detect 10 ng HA tag antigen. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

 

Figure 2. Western blot: decreasing amounts of HA-tagged mouse recombinant CD40 ligand (25 – 35 kDa) detected with PtX™ Mouse Anti-HA Tag at 1: 1 000 and an HRP conjugated anti-mouse secondary antibody.

 

PtX™ | Plant-Based Expression

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single-use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.

Visit our Technology page to learn more.

Important!

  • For Research Use Only.
  • Not for diagnostic or therapeutic use.
  • Not for resale without express authorization.
  • Trademark.

Feel free to contact our trusted distribution partners directly if you want to order this product.

 

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