Human Anti-Alpha Tubulin (F2C) (HRP)

Recombinant human monoclonal antibody against Human Alpha Tubulin, fused at the genetic level to HRP reporter enzyme. This product is produced in Nicotiana benthamiana plants and is suitable for Western Blot and ELISA applications.

100 µg      |     Catalogue Number: CBT_A0054

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Alpha-tubulin protein is a globular protein that consists of approximately 450 amino acid residues and forms heterodimers with beta-tubulin to produce microtubules, the cytoskeletal filaments found in eukaryotic cells. The amino acid sequence of alpha-tubulin is highly conserved across eukaryotes, and contains a GTP-binding domain, which is important for regulating the assembly and disassembly of microtubules. Alpha-tubulin plays a crucial role in cell division, intracellular transport, and the maintenance of cell shape and polarity. Mutations in the alpha-tubulin gene have been linked to various diseases, including developmental disorders and cancer. The study of alpha-tubulin and its interactions with other proteins has important implications for understanding the basic biology of cells and the development of new therapies for human diseases.

Additional information

Available Unit Size

0.1mg, 1mg, 10mg, 100mg, 200mg

Key Benefits

This product is a recombinant monoclonal antibody that offers several benefits including the following:

  • Enhanced reproducibility associated with HRP fusion 
  • Reduced Background Noise 
  • Time and Cost Efficiency 
  • Compatibility with Chemiluminescent and Colorimetric Detection 
  • High batch-to-batch consistency 
  • No animals or animal products are used in the production of this antibody. 
  • BSA and Azide free. 

For more information on plant-made, recombinant monoclonal antibodies click here.

Datasheet

Product Name PtX™ Human Anti-Alpha Tubulin (F2C) (HRP)
Catalogue Number CBT_A0054
Expression Host Nicotiana benthamiana plants
Clonality Monoclonal, recombinant
Species and Isotype Human IgG1
Tag None
Reporter Protein Horseradish Peroxidase (HRP)
Description This product is a full-length Human IgG1 antibody specific to Human Alpha tubulin protein, fused at the genetic level to the HRP reporter enzyme. It was produced recombinantly in Nicotiana benthamiana plants via Agrobacterium tumefaciens mediated infiltration.
Target name Human Alpha Tubulin
Verified Applications Western blot, ELISA
Recommended Dilutions Western blot (1: 1000 – 1: 4000)
ELISA (1: 500 – 1: 200 000)
Concentration 1.0 mg/ml
Form Liquid
Colour Clear to light pink/red
Preparation Ready to use
Storage Short-term (up to one week): 2 – 8 °C
Long term: Aliquot and store at – 20 °C
Store immediately. Aliquot and avoid multiple freeze-thaw cycles.
Storage Buffer 0.1 M Phosphate Buffered Saline, pH 7.4
Preservative: None
Purification Notes This product was purified using Protein A affinity chromatography.
Purity ≥ 95% as determined by SDS-PAGE
General Notes If for any reason the product does not perform as specified, please contact our scientific support team for assistance by emailing sales@capebiologix.com.

Images

Figure 1. ELISA Dose Response curve using PtX™ Human Anti-Alpha Tubulin (HRP) Antibody from 2.0 – 0,0000256 ng/uL to detect 25 ng TUBA1A antigen. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot of decreasing amounts of Human Alpha (α) Tubulin (~50kDa) detected with PtX™ Human Anti-Alpha Tubulin (HRP) at 1: 4 000.

 

PtX™ | Plant-Based Expression

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single-use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.

Visit our Technology page to learn more.

Important!

  • For Research Use Only.
  • Not for diagnostic or therapeutic use.
  • Not for resale without express authorization.
  • Trademark.

Feel free to contact our trusted distribution partners directly if you want to order this product.

 

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