Mouse Anti-IFN Gamma (Biotin)

Mouse Anti-IFN Gamma (Biotin)

This product is a full-length biotinylated Mouse IgG2a recombinant antibody specific to interferon gamma (IFNγ). It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.

100 µg      |     Catalogue Number: CBT_A0012[B]

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Interferon-Gamma (IFN-γ) is a pleiotropic cytokine and the only member of the type II IFN family, which differs structurally and functionally from other types of IFNs. IFN-γ is a homodimer synthesized by non-covalent association of two 146 amino acid subunits in an antiparallel manner, forming a mature protein of approximately 33 kDa. Antigens, mitogens and cytokines, e.g. IL-12, IL-15 and IL-18, induce production of IFN-γ by T lymphocytes and natural killer cells. Once released, it acts as macrophage activation factor through binding of IFN-γ receptor I and II and subsequent activation of the JAK/STAT pathway. The inhibitory effects of IFN-γ on viral replication were originally reported in 1965 and its role in host response to microorganisms has been well established. IFN-γ also plays an important role in cancer immune surveillance, promoting tumor recognition and immunity.

The biotin labelling of this antibody ensures strong and stable interaction with avidin or streptavidin, making it suitable for a wide range of downstream applications, including Western blotting and ELISA.

Additional information

Available Unit Size

0.1mg, 1mg, 10mg, 100mg, 200mg

Key Benefits

This product is a recombinant monoclonal antibody that offers several benefits including the following: 

  • Enhanced signal intensity and sensitivity
  • Reduced background noise
  • Compatibility with multiple species and antibody isotypes
  • Minimized steric hindrance.
  • Batch-to-batch consistency
  • This product is plant-made, no animals or animal products are used in the manufacture of this antibody.
  • BSA and Azide free

For more information on plant-made, recombinant monoclonal antibodies click here. 

Datasheet

Product Name PtX™ Mouse Anti-Interferon Gamma (Biotin)
Catalogue Number CBT_A0012[B]
Expression Host Nicotiana benthamiana plants
Clonality Monoclonal, recombinant
Species and Isotype Mouse IgG2a
Tag Biotin
Reporter Protein None
Description This product is a full-length Mouse IgG2a recombinant antibody specific to interferon gamma (IFNγ). It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.
Target Name Interferon Gamma (IFNγ)
Verified Applications Western blot
Dilution Range Western blot (1: 1 000 – 1: 3 000)
Tested Species Reactivity     N/A
Concentration                   1 mg/ml
Form Liquid
Colour Clear
Preparation Ready to use
Storage Short term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze thaw cycles.
Storage Buffer 0.1M Phosphate Buffered Saline, pH 7.4
Purification Notes This product was purified using Protein A- affinity chromatography.
Purity ≥ 95 % as determined by SDS-PAGE
General Notes If for any reason the product does not perform as specified, please contact our scientific support team for assistance by emailing techsupport@capebiologix.com.

For Research Use only, unless otherwise indicated.

Images

Figure 1. Western blot: decreasing amounts of Interferon gamma (IFNγ) Protein (double bands at 33 kDa (homodimer) and 17 kDa (monomer) detected with PtX™ Mouse Anti-Interferon Gamma (Biotin) Antibody at 1: 2 500 and HRP conjugated Streptavidin at 1: 5 000.

 

PtX™ | Plant-Based Expression

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single-use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.

Visit our Technology page to learn more.

Important!

  • For Research Use Only.
  • Not for diagnostic or therapeutic use.
  • Not for resale without express authorization.
  • Trademark.

Feel free to contact our trusted distribution partners directly if you want to order this product.

 

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