Mouse Anti-Beta Tubulin

Recombinant mouse monoclonal antibody against Beta Tubulin. Produced in Nicotiana benthamiana plants and suitable for WB, ELISA and Immunocytochemistry applications.

100 µg      |     Catalogue Number: CBT_A0041

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Tubulin is a major component of microtubules, the scaffolding of the eukaryotic cytoskeleton. It plays multiple roles in the maintenance of cell structure, intracellular vesicle and organelle transport, and cell division (mitosis and meiosis). Microtubules are formed by polymerization of alpha and beta tubulin isotypes, and the addition of proteins referred to as microtubule-associated proteins (MAPs). It has a molecular weight of 50 kDa and is composed of a double β-sheet core, enclosed within α-helices. Beta tubulin is a highly conserved, negatively charged protein, which has made it a popular target for anti-cancer drugs, aimed at disrupting microtubule dynamics and cell division. Anti-beta tubulin antibodies are commonly used as loading controls for immunoblotting to verify that uniform quantities of protein have been loaded across wells.

Additional information

Available Unit Size

0.1mg, 1mg, 10mg, 100mg, 200mg

Key Benefits

This product is a recombinant monoclonal antibody that offers several benefits including the following:

  • Batch-to-batch consistency.
  • This product is plant-made, no animals or animal products are used in the manufacture of this protein.
  • BSA and Azide free.

For more information on plant-made, recombinant monoclonal antibodies click here.

Datasheet

Product Name PtX™ Mouse Anti-Beta Tubulin (S11B) Recombinant Antibody
Catalogue Number CBT_A0041
Expression Host Nicotiana benthamiana plants
Clonality Monoclonal, recombinant
Species and Isotype Mouse IgG2a
Tag None
Reporter Protein None
Description This product is a full-length Mouse IgG2a recombinant antibody specific to Beta Tubulin. It was produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens-mediated infiltration.
Target Name Beta Tubulin
Verified Applications Western blot, ELISA, Immunocytochemistry
Dilution Range

Western blot (1: 5 000 – 1: 10 000)

ELISA (1: 65 000 – 1: 1 000 000)

Immunocytochemistry (1:100-1:300)
Tested Species Reactivity     Human, Mouse
Concentration                   1 mg/ml
Form Liquid
Colour Clear
Preparation Ready to use
Storage

Short term (up to one week): 2 – 8 °C

Long term: Aliquot and store at – 20 °C

Store immediately. Aliquot and avoid multiple freeze thaw cycles.
Storage Buffer

0.1   M Phosphate Buffered Saline, pH 7.4

Preservative: None
Purification Notes This product was purified using Protein A- affinity chromatography.
Purity 95 % as determined by SDS-PAGE
General Notes

If for any reason the product does not perform as specified, please contact our scientific support team for assistance by emailing techsupport@capebiologix.com.

For Research Use only, unless otherwise indicated.

Images

Figure 1. ELISA Dose Response curve using PtX™ Mouse Anti-Beta Tubulin (S11B) to detect coated Recombinant Human Tubulin beta chain Antigen at 0.25 ng/µl and increasing concentrations of antibody. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot of decreasing amounts of recombinant Human beta chain antigen (50-70 kDa) detected with PtX™ Mouse Anti-Beta Tubulin (S11B) Antibody at 1: 10 000 and detected with a HRP conjugated anti-mouse secondary antibody at 1: 10 000.

Figure 3. Immunocytochemistry: HeLa cells were plated at 200 000 cells/ well in 6-well plates on coverslips and allowed to adhere. Following fixation and blocking, cells were incubated with 1:200 dilution of Cape Biologix PtXTM Mouse Anti-β-Tubulin primary antibody (Cat# CBT_A0041), and 1:300 dilution of a commercial Anti-Mouse Alexa Fluor® 488 conjugated commercial secondary antibody. Images were taken on a Zeiss LSM780 with ELYRA PS1 platform confocal microscope (20X) at the Stellenbosch University CAF unit. Thank you to Prof Georgia Schafer (ICGEB) for kindly donating the HeLa cells and Mrs Lize Engelbrecht for her outstanding assistance.

PtX™ | Plant-Based Expression

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single-use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.

Visit our Technology page to learn more.

Important!

  • For Research Use Only.
  • Not for diagnostic or therapeutic use.
  • Not for resale without express authorization.
  • Trademark.

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