Human Anti-N (N12.8) (HRP)

Recombinant mouse monoclonal antibody against SARS-CoV-2 Nucleocapsid protein. This product is produced in Nicotiana benthamiana plants for use in Western blot and ELISA applications.

100 µg      |     Catalogue Number: CBT_A0067

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The SARS-CoV-2 nucleocapsid (N) protein is the most abundantly expressed protein of the coronavirus. The N protein is composed of two protein domains connected by an intrinsically disordered region (IDR), known as the linker region. It binds to RNA to form helical ribonucleoprotein (RNP) structures during packaging of the genome into the viral capsid, while also regulating RNA synthesis during replication and transcription. N protein, along with the coronavirus membrane protein (M), facilitates viral assembly, while also modulating host cell metabolism and manipulating the cell cycle. Due to the strong immunogenicity and conserved nature of the coronavirus nucleocapsid, N proteins have value in diagnostic and vaccine development.

Additional information

Available Unit Size

0.1mg, 1mg, 10mg, 100mg, 200mg

Key Benefits

This product is a recombinant monoclonal antibody that offers several benefits including the following:

  • Batch-to-batch consistency.
  • This product is plant-made, no animals or animal products are used in the manufacture of this protein.
  • Directly fused to HRP at genetic level- increased reproducibility.
  • BSA and Azide free.

For more information on plant-made, recombinant monoclonal antibodies click here.

Datasheet

Product Name PtX™ Human Anti-SARS CoV-2 Nucleocapsid Protein (N12.8) (HRP) Recombinant Antibody
Catalogue Number CBT_A0067
Expression Host Nicotiana benthamiana plants
Clonality Monoclonal, recombinant
Species and Isotype Human IgG1
Tag None
Reporter Protein Horseradish Peroxidase (HRP)
Description Recombinant human monoclonal antibody against SARS-CoV-2 Nucleocapsid protein, fused at the genetic level to HRP reporter enzyme. This protein was produced via Agrobacterium tumefaciens infiltration of Nicotiana benthamiana plants.
Verified Applications Western blot (1: 500 – 1: 1 000)
ELISA (1: 1 000 – 1: 15 000)
Concentration 1.0 mg/ml
Form Liquid
Colour Clear with faint red tinge
Preparation Ready to use
Storage Short-term (up to one week): 2 – 8 °C
Long term: Aliquot and store at – 20 °C
Store immediately. Aliquot and avoid multiple freeze-thaw cycles.
Storage buffer 0.1 M Phosphate Buffered Saline, pH 7.4.
Preservative: 50% Glycerol
Purification notes This product was purified using Protein A affinity chromatography.
Purity ≥ 90 % as determined by SDS-PAGE
94.20% as determined by Mass Spectrometry.
General notes If for any reason the product does not perform as specified, please contact our scientific support team for assistance by emailing techsupport@capebiologix.com.

For Research Use only, unless otherwise indicated.

Images

Figure 1. ELISA Dose Response curve showing PtX™ Human Anti-N (N12.8) (HRP) Antibody used from 0,003 – 50 ng/uL to detect 50 ng SARS-CoV-2 Nucleocapsid protein. Experiments were performed in triplicate, with error bars representing standard deviation (SD).

Figure 2. Western blot showing decreasing amounts of SARS-CoV-2 Nucleocapsid protein (46 kDa) detected with PtX™ Human Anti-N (N12.8) (HRP) Antibody at 1:1000.

PtX™ | Plant-Based Expression

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single-use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.

Visit our Technology page to learn more.

Important!

  • For Research Use Only.
  • Not for diagnostic or therapeutic use.
  • Not for resale without express authorization.
  • Trademark.

Feel free to contact our trusted distribution partners directly if you want to order this product.

 

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