PtX™ Rabbit Anti-IFN-γ

Recombinant rabbit monoclonal antibody against Interferon gamma, produced in Nicotiana benthamiana plants. Suitable for Western Blot applications.

20 µg Sample Size     |     Catalogue Number: CBT_A0004

Download: SDS / Datasheet

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Interferon gamma (IFN-γ) is a pleiotropic cytokine and the only member of the type II IFN family, which differs structurally and functionally from other types of IFNs. IFN-γ is a homodimer synthesized by non-covalent association of two 146 amino acid subunits in an antiparallel manner, forming a mature protein of approximately 33 kDa. Antigens, mitogens, and cytokines e.g., IL-12, IL-15, and IL-18 induce production of IFN-γ by T lymphocytes and natural killer cells. Once released it acts as a macrophage activation factor through binding of IFN-γ receptor I and II, and subsequent activation of the JAK/STAT pathway. The inhibitory effects of IFN-γ on viral replication were originally reported in 1965 and its role in host response to microorganisms have been well established. IFN-γ also plays an important role in cancer immune surveillance, promoting tumor recognition and immunity.

Additional information

Available Unit Size

0.1mg, 1mg, 10mg, 100mg, 200mg

Key Benefits

This product is a plant-made, recombinant monoclonal antibody which offers several benefits including the following:

  • Capable of binding both subunits of Interferon gamma.
  • No animals or animal products are used in the development of this protein.
  • BSA and Azide free.

For more information on plant-made, recombinant monoclonal antibodies click here.

Datasheet

Product Name PtX™ Rabbit Anti-Interferon Gamma Recombinant Antibody
Catalogue Number CBT_A0004
Expression Host Nicotiana benthamiana plants
Clonality Monoclonal, recombinant
Species and Isotype Rabbit IgG1
Tag None
Reporter Protein None
Description This is a recombinant antibody targeted to IFN-γ cytokine. It is produced in Nicotiana benthamiana plants via Agrobacterium tumefaciens mediated infiltration.
Verified Applications Western blot (1:2 000 – 1:7 500)
Concentration 1.0 mg/ml
Form Liquid
Colour Clear
Preparation Ready to use
Storage Short-term (up to one week): 2 – 8 °C
Long-term: Aliquot and store at – 20 °C
Store immediately. Aliquot and avoid multiple freeze thaw cycles.
Storage buffer Phosphate Buffered Saline (0.1 M), pH 7.4. Preservative: none
Purification notes This product was purified using Protein A affinity chromatography.
Purity ≥ 90 % as determined by SDS-PAGE.
General notes If for any reason the product does not perform as specified, please contact our scientific support team for assistance by emailing sales@capebiologix.com.

Images

Figure 1: Western blot analysis of PtX™ Rabbit Anti-IFN-γ. Lanes 2 – 6: Varying amounts of antigen (IFN-γ) were run on the SDS-PAGE. Separated bands were transferred to the membrane and PtX™ Rabbit Anti-IFN-γ (1:2 000) was used to detect the antigen. The two bands of IFN-γ were visualized following the addition of anti-rabbit secondary antibody with HRP and substrate. Our antibody was able to detect antigen amounts upwards of 30ng (Lane 6).

PtX™ | Plant-Based Expression

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single-use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.

Visit our Technology page to learn more.

Important!

  • For Research Use Only.
  • Not for diagnostic or therapeutic use.
  • Not for resale without express authorization.
  • Trademark.

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