Human Anti-TNF-Alpha

Recombinant human monoclonal antibody against Tumor Necrosis Factor Alpha cytokine. This product is produced in Nicotiana benthamiana plants and is suitable for Western Blot and ELISA applications.

100 µg      |     Catalogue Number: CBT_A0013

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TNF-Alpha (tumor necrosis factor alpha) is a pro-inflammatory cytokine that is produced and released by various cell types, including T-cells, macrophages, and monocytes. TNF-α acts by binding to specific receptors on the surface of target cells, which leads to the activation of signaling pathways that result in inflammation, cell death, and tissue destruction. It also plays a role in the development of cancer and the progression of autoimmune diseases such as rheumatoid arthritis and Crohn's disease. Targeting TNF-α has become a major therapeutic strategy for treating cancer and inflammatory diseases. The high specificity and binding affinity of our PtX™ Human Anti-Tumor Necrosis Factor Alpha Recombinant Antibody makes it an ideal tool for researchers studying TNF alpha-mediated signaling pathways and its potential as a therapeutic target. This antibody can be used in various applications including Western blotting and ELISA. This product is suitable for research use only and not for use in humans.

Additional information

Available Unit Size

0.1mg, 1mg, 10mg, 100mg, 200mg

Key Benefits

This product is a recombinant monoclonal antibody that offers several benefits including the following:

  • Batch-to-batch consistency.
  • No animals or animal products are used in the development of this protein.
  • BSA and Azide free.

For more information on plant-made, recombinant monoclonal antibodies click here.

Datasheet

Product Name PtX™ Human Anti-Tumour Necrosis Factor Alpha Recombinant Antibody
Catalogue Number CBT_A0013
Expression Host Nicotiana benthamiana plants
Clonality Monoclonal
Species and Isotype Human IgG1
Tag None
Reporter Protein None
Description Recombinant human monoclonal antibody against TNF-Alpha cytokine, produced via Agrobacterium tumefaciens infiltration of Nicotiana benthamiana plants.
Verified Applications Western blot, ELISA
Recommended dilutions Western blot (1: 2 000 – 1: 20 000)
ELISA (1: 1 000 – 1: 10 000)
Concentration 1.0 mg/ml
Form Liquid
Colour Clear
Preparation Ready to use
Storage Short-term (up to one week): 2 – 8 °C
Long term: Aliquot and store at – 20 °C
Store immediately. Aliquot and avoid multiple freeze-thaw cycles.
Storage buffer 0.1  M Phosphate Buffered Saline, pH 7.4.
Preservative: none
Purification notes This product was purified using Protein A affinity chromatography.
Purity ≥ 90 % as determined by SDS-PAGE.
98.28 % as determined by Mass Spectrometry.
General notes If for any reason the product does not perform as specified, please contact our scientific support team for assistance by emailing sales@capebiologix.com.

Images

Figure 1: Functionality Testing of PtX™ Human Anti-TNF-Alpha ELISA Dose Response curve showing increasing absorbance with increasing amounts 96 well plate coated with 0.4 ng/µl of TNF alpha antigen.

Figure 2:  Western blot analysis of PtX™ Human Anti-TNF-Alpha. Lanes 2 – 6: Varying amounts of TNF- Alpha were run on an SDS-PAGE gel. Separated bands were transferred to the membrane and PtX™ Human Anti-TNF-α (1: 5 000) was used to detect the antigen. The single band of TNF-Alpha cytokine was visualized following the addition of anti-human secondary antibody with HRP and substrate. Our antibody was able to detect antigen amounts upwards of 25 ng (Lane 5).

PtX™ | Plant-Based Expression

The biotechnology behind the development and manufacture of this product, transient plant-based expression, effectively turns Nicotiana benthamiana plants into single-use, biodegradable bioreactors. Infiltrating this plant species with Agrobacterium tumefaciens cells, transformed with our proprietary plant expression vectors, allows rapid production of a wide variety of recombinant proteins. Plants are harvested and proteins extracted by various stages of filtration, clarification, purification, and polishing – resulting in a highly functional final product.

Visit our Technology page to learn more.

Important!

  • For Research Use Only.
  • Not for diagnostic or therapeutic use.
  • Not for resale without express authorization.
  • Trademark.

Feel free to contact our trusted distribution partners directly if you want to order this product.

 

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